| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX47 |
| Uniprot No | Q9H0S4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455aa |
| 氨基酸序列 | MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR |
| 分子量 | 77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DDX47蛋白的参考文献概述：
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**1. 标题**: *"Recombinant Human DDX47 Exhibits RNA Unwinding Activity and Facilitates Ribosome Biogenesis in Vitro"*
**作者**: Linder, P., et al.
**摘要**: 本研究表达了重组人DDX47蛋白并验证其RNA解旋酶活性。通过体外实验证明，DDX47能够结合并解旋双链RNA结构，且在核糖体RNA前体加工过程中可能参与核仁的RNA代谢调控。
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**2. 标题**: *"DDX47 Promotes Tumor Progression via Modulating Alternative Splicing in Hepatocellular Carcinoma"*
**作者**: Wang, Y., et al.
**摘要**: 通过重组DDX47蛋白的功能实验，作者发现该蛋白在肝癌细胞中高表达，并调控特定mRNA的选择性剪接，进而促进肿瘤细胞侵袭和化疗耐药性。
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**3. 标题**: *"Structural Insights into the ATP-Dependent Mechanism of Human DDX47 Helicase"*
**作者**: Côté, F., et al.
**摘要**: 本研究利用重组DDX47蛋白解析了其结合ATP和RNA时的晶体结构，揭示了其通过ATP水解驱动构象变化的工作机制，为靶向DDX47的药物设计提供了结构基础。
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**备注**: 以上为模拟文献，实际研究中需通过PubMed或Google Scholar以“DDX47”、“recombinant protein”等关键词检索真实文献。部分研究可能侧重DDX47的生理/病理功能，而非重组蛋白技术本身。
**Background of Recombinant Human DDX47 Protein**
DDX47. a member of the DEAD-box helicase family, is an RNA-binding protein involved in diverse RNA metabolic processes. DEAD-box proteins, characterized by a conserved Asp-Glu-Ala-Asp (DEAD) motif, play critical roles in RNA unwinding, splicing, ribosome biogenesis, and RNA transport. Human DDX47. located in the nucleolus and nucleus, is implicated in pre-mRNA processing, particularly in the early stages of spliceosome assembly, and interacts with components of the U2 snRNP complex. Its helicase activity facilitates RNA structural remodeling, aiding in splice site selection and maturation of ribosomal RNA.
Recombinant human DDX47 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purified recombinant DDX47 retains ATP-dependent RNA helicase activity, making it a valuable tool for investigating RNA-protein interactions, spliceosome dynamics, and ribosome biogenesis mechanisms. Dysregulation of DDX47 has been linked to cancers and viral infections, as it may influence cell proliferation and viral RNA processing. Studies using recombinant DDX47 also explore its role in stress granule formation and cellular responses to RNA damage. Current research focuses on elucidating its structural domains, substrate specificity, and potential as a therapeutic target in diseases marked by aberrant RNA metabolism.
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