| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX50 |
| Uniprot No | Q9BQ39 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-737aa |
| 氨基酸序列 | MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD |
| 分子量 | 109 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX50蛋白的3篇参考文献示例，内容基于科学文献常见研究方向合理推测，供参考：
---
1. **文献名称**: "Recombinant Human DDX50 Exhibits RNA Helicase Activity and Antiviral Immune Response Regulation"
**作者**: Li, X., et al.
**摘要**: 研究报道了在大肠杆菌中成功表达重组人DDX50蛋白，并验证其ATP依赖的RNA解旋酶活性。进一步实验表明，DDX50通过结合病毒RNA并激活RIG-I信号通路，增强I型干扰素产生，提示其在抗病毒先天免疫中的关键作用。
2. **文献名称**: "Structural Analysis of Recombinant DDX50 Reveals a Novel Role in Ribosome Biogenesis"
**作者**: Müller, S., et al.
**摘要**: 通过X射线晶体学解析了重组人DDX50蛋白的C端结构域，发现其与核糖体RNA加工复合物相互作用。功能实验显示，DDX50通过调控28S rRNA的成熟过程影响核糖体组装，进而参与细胞增殖调控。
3. **文献名称**: "DDX50 Interacts with TRIM25 to Modulate RNA Virus Sensing Pathways"
**作者**: Park, J.H., et al.
**摘要**: 本研究利用重组人DDX50蛋白进行免疫共沉淀实验，发现其与TRIM25蛋白直接结合，共同促进RIG-I的泛素化修饰。结果表明，DDX50通过增强宿主对RNA病毒(如流感病毒)的识别能力，优化先天免疫应答。
---
注：以上文献为模拟示例，实际引用需检索权威数据库(如PubMed)。如需真实文献，可结合关键字“Human DDX50”或“DEAD-box helicase 50”在Google Scholar等平台查找。
Recombinant human DDX50 protein is a genetically engineered form of the DDX50 helicase, a member of the DEAD-box family of ATP-dependent RNA helicases. DDX50. also known as GEDE4 or HEL-117. plays essential roles in RNA metabolism, including ribosome biogenesis, RNA processing, and transcriptional regulation. Structurally, it contains conserved DEAD-box motifs involved in ATP hydrolysis and RNA binding, enabling its function in unwinding RNA secondary structures or remodeling RNA-protein complexes.
DDX50 is implicated in innate immune responses, particularly antiviral defense, by interacting with pathways such as RIG-I to enhance interferon production. Dysregulation of DDX50 has been linked to cancers and viral infections, highlighting its biological and clinical relevance. Recombinant DDX50 is typically produced in expression systems like *E. coli* or mammalian cells, followed by purification for functional studies. Researchers use it to investigate helicase mechanisms, RNA-protein interactions, or screen for inhibitors targeting its enzymatic activity. Its recombinant form allows scalable production for structural analysis (e.g., crystallography) and therapeutic exploration, such as antiviral drug development. Studies on DDX50 also contribute to understanding cellular stress responses and ribosomal RNA maturation, bridging RNA biology with disease mechanisms.
×
