| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX55 |
| Uniprot No | Q8NHQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207aa |
| 氨基酸序列 | MKPQRNTADLLPKLKSMALADRAVFEKGMKAFVSYVQAYAKHECNLIFRLKDLDFASLARGFALLRMPKMPELRGKQFPDFVPVDVNTDTIPFKDKIREKQRQKLLEQQRREKTENEGRRKFIKNKAWSKQKAKKEKKKKMNEKRKREEGSDIEDEDMEELLNDTRLLKKLKKGKITEEEFEKGLLTTGKRTIKTVDLGISDLEDDC |
| 分子量 | 50.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX55蛋白的模拟参考文献(文献为虚构，仅供示例参考)：
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1. **"Expression and Functional Characterization of Recombinant Human DDX55 in HEK293 Cells"**
*作者：Zhang L. et al.*
**摘要**：本研究成功在HEK293细胞中表达了重组人DDX55蛋白，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，DDX55具有ATP依赖的RNA解旋酶活性，且在体外促进核糖体RNA前体的加工。
2. **"Structural Insights into the RNA Unwinding Mechanism of DDX55 Helicase"**
*作者：Kimura T. et al.*
**摘要**：通过X射线晶体学解析了重组DDX55的ATP结合结构域三维结构，揭示了其与RNA底物结合的分子机制。研究发现，DDX55通过保守的DEAD-box模体调控RNA解旋过程中的构象变化。
3. **"DDX55 Promotes Cancer Cell Proliferation via Regulating MYC mRNA Stability"**
*作者：Chen X. et al.*
**摘要**：文章发现重组DDX55在结直肠癌细胞中过表达，并通过结合MYC mRNA的3'UTR增强其稳定性，从而促进肿瘤生长。敲低DDX52显著抑制体内外肿瘤增殖。
4. **"Viral Infection Induces DDX55 Redistribution to Facilitate Viral RNA Replication"**
*作者：Wang H. et al.*
**摘要**：研究表明，流感病毒感染后，重组DDX55从细胞核转位至胞质，与病毒RNP复合物相互作用，通过解旋病毒RNA二级结构促进病毒基因组复制。
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*注：以上文献为示例性质，实际研究请参考真实数据库(如PubMed)。建议通过关键词“DDX55”“DEAD-box”“RNA helicase”等检索最新文献。*
Recombinant human DDX55 protein is a genetically engineered version of the endogenous DDX55. a member of the DEAD-box RNA helicase family. Characterized by its conserved Asp-Glu-Ala-Asp (DEAD) motif, DDX55 plays a critical role in RNA metabolism, including ribosome biogenesis, pre-mRNA processing, and translational regulation. The protein, encoded by the DDX55 gene on human chromosome 12. contains approximately 600 amino acids and exhibits ATP-dependent RNA unwinding activity. It localizes primarily to the nucleolus and is implicated in rRNA processing and cell cycle progression. Studies suggest its involvement in cellular proliferation, differentiation, and potential roles in cancer progression or viral infection by interacting with viral RNAs or proteins. Recombinant DDX55 is typically produced in bacterial (e.g., E. coli) or mammalian expression systems, enabling functional studies of its helicase activity, RNA-binding properties, and interactions with biological partners. Its availability facilitates research into ribosome assembly defects, RNA-related diseases, and antiviral drug development. Applications also include biochemical assays, structural analysis, and screening for DDX55-targeted therapeutics in oncology and virology.
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