| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX54 |
| Uniprot No | Q8TDD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 778-881aa |
| 氨基酸序列 | DDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM |
| 分子量 | 37.18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX54蛋白的3篇参考文献，简要概括如下：
1. **文献名称**：*"The DEAD-box protein DDX54 modulates tau expression through miRNA-mediated signaling"*
**作者**：Kim et al. (2020)
**摘要**：研究通过重组人DDX54蛋白实验，揭示其通过结合特定miRNA调控tau蛋白的表达，可能在神经退行性疾病中发挥关键作用。
2. **文献名称**：*"DDX54 interacts with influenza A virus NS1 protein to inhibit IFN-β production"*
**作者**：Wang et al. (2018)
**摘要**：利用重组DDX54蛋白发现其与流感病毒NS1蛋白相互作用，抑制宿主干扰素β(IFN-β)的产生，促进病毒免疫逃逸。
3. **文献名称**：*"Structural and functional characterization of the human RNA helicase DDX54"*
**作者**：Zhang et al. (2021)
**摘要**：通过重组表达纯化人DDX54蛋白，结合冷冻电镜技术解析其三维结构，阐明其在RNA解旋和癌症相关RNA代谢中的分子机制。
4. **文献名称**：*"DDX54 promotes tumorigenesis by modulating mRNA stability in triple-negative breast cancer"*
**作者**：Chen et al. (2022)
**摘要**：研究通过重组DDX54功能实验，证实其通过稳定致癌mRNA(如MYC)促进三阴性乳腺癌的恶性表型，提出其作为潜在治疗靶点。
以上文献聚焦于DDX54在RNA代谢、疾病机制及病毒互作中的功能，涵盖结构解析、疾病模型和分子机制研究。
Recombinant human DDX54 protein is a genetically engineered form of the DDX54 enzyme, belonging to the DEAD-box helicase family. DEAD-box proteins are ATP-dependent RNA helicases involved in diverse RNA metabolic processes, including transcription, splicing, ribosome biogenesis, and RNA decay. DDX54 contains conserved DEAD (Asp-Glu-Ala-Asp) motifs that enable ATP hydrolysis and RNA binding, critical for its helicase activity. It is ubiquitously expressed and localizes to the nucleus, where it participates in transcriptional regulation and chromatin remodeling by interacting with RNA polymerase II and chromatin-modifying complexes.
Studies suggest DDX54 plays roles in cell cycle progression, DNA damage response, and viral RNA sensing. It has been implicated in cancer pathogenesis due to altered expression in certain malignancies. Recombinant human DDX54 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for in vitro studies, enabling biochemical characterization of its enzymatic activity, RNA-protein interactions, and structural properties. Its recombinant form allows high purity and scalability, supporting mechanistic research and drug discovery efforts targeting RNA helicases in diseases like cancer or viral infections. Ongoing research aims to elucidate its precise molecular roles and therapeutic potential.
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