| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX59 |
| Uniprot No | Q5T1V6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 氨基酸序列 | MFVPRSLKIKRNANDDGKSCVAKIIKPDPEDLQLDKSRDVPVDAVATEAATIDRHISESCPFPSPGGQLAEVHSVSPEQGAKDSHPSEEPVKSFSKTQRWAEPGEPICVVCGRYGEYICDKTDEDVCSLECKAKHLLQVKEKEEKSKLSNPQKADSEPESPLNASYVYKEHPFILNLQEDQIENLKQQLGILVQGQEVTRPIIDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAFLLPVIMRALFESKTPSALILTPTRELAIQIERQAKELMSGLPRMKTVLLVGGLPLPPQLYRLQQHVKVIIATPGRLLDIIKQSSVELCGVKIVVVDEADTMLKMGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKRISIHSEKSQIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQIGRVGRLGQNGTAITFINNNSKRLFWDIAKRVKPTGSILPPQLLNSPYLHDQKRKEQQKDKQTQNDLVTGANLMDIIRKHDKSNSQK |
| 分子量 | 95.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX59蛋白的参考文献示例(注：部分文献为假设性示例，具体内容需根据实际文献调整)：
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1. **"Mutations in DDX59 cause a novel subtype of autosomal recessive ectodermal dysplasia"**
*作者：Weedon MN, et al. (2022)*
**摘要**：该研究报道了DDX59基因突变导致常染色体隐性遗传性外胚层发育不良，并通过重组人DDX59蛋白实验验证了突变体对ATP酶活性和RNA解旋功能的破坏，揭示了其在细胞核内RNA代谢中的关键作用。
2. **"Biochemical characterization of recombinant human DDX59 helicase activity"**
*作者：Smith J, et al. (2020)*
**摘要**：通过大肠杆菌表达系统成功纯化重组人DDX59蛋白，证明其具有ATP依赖的RNA解旋酶活性，并发现其在pre-rRNA加工过程中可能参与特定RNA结构的重塑。
3. **"DDX59 interacts with small nucleolar RNAs (snoRNAs) and regulates ribosomal biogenesis"**
*作者：Lee S, et al. (2019)*
**摘要**：通过免疫共沉淀和体外重组蛋白实验，发现DDX59与snoRNAs直接结合，并通过调控核糖体RNA前体(pre-rRNA)的剪切过程影响核糖体生物合成，提示其在细胞增殖中的潜在作用。
4. **"Structural insights into the catalytic mechanism of human DDX59 protein"**
*作者：Chen X, et al. (2021)*
**摘要**：利用冷冻电镜技术解析了重组人DDX59蛋白的晶体结构，揭示了其DEAD-box结构域与RNA结合的分子机制，并提出了其ATP水解偶联RNA解旋的功能模型。
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**注意**：以上文献名称及内容为综合DDX59蛋白研究领域的常见方向模拟生成，实际引用需以具体出版物为准。建议通过PubMed、Web of Science等数据库检索最新文献。
Recombinant human DDX59 protein is a genetically engineered form of the DDX59 helicase, a member of the DEAD-box RNA helicase family involved in RNA metabolism. DDX59 plays critical roles in ribosomal RNA (rRNA) processing, ribosome biogenesis, and possibly in the regulation of gene expression. It contains conserved helicase core domains and unique N/C-terminal extensions that mediate interactions with RNA and other proteins. Mutations in the DDX59 gene have been linked to orofaciodigital syndrome type V (OFD5), a rare genetic disorder characterized by craniofacial anomalies and skeletal defects, suggesting its essential role in developmental processes.
Recombinant DDX59 is typically expressed in bacterial or mammalian systems for biochemical and functional studies. Researchers use it to investigate helicase activity, RNA-binding properties, and molecular mechanisms underlying DDX59-associated diseases. Its recombinant form enables detailed structural analyses, drug screening for therapeutic interventions, and exploration of its role in ribosome assembly pathways. Recent studies also propose connections between DDX59 dysfunction and cellular stress responses, highlighting its broader implications in RNA quality control. While much remains unclear about its full range of functions, recombinant DDX59 serves as a vital tool for deciphering RNA helicase biology and disease pathology.
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