| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEDD2 |
| Uniprot No | Q8WXF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185aa |
| 氨基酸序列 | MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLLELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSANSQQGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPAQTFL |
| 分子量 | 47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于 **重组人DEDD2蛋白** 的模拟参考文献(注：部分信息为示例性构建，实际文献需根据数据库检索确认)：
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### 1. **《Recombinant expression and functional characterization of human DEDD2 in apoptosis regulation》**
**作者**: Zhang Y, et al.
**摘要**:
研究通过大肠杆菌系统成功表达并纯化了重组人DEDD2蛋白，发现其通过促进caspase-8的激活增强TNF-α诱导的凋亡。进一步实验表明DEDD2与FADD相互作用，调控死亡受体信号通路。
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### 2. **《DEDD2 modulates autophagy and tumorigenesis by interacting with mTORC1》**
**作者**: Li X, Wang Q, et al.
**摘要**:
研究利用哺乳动物细胞系表达重组DEDD2.发现其通过与mTORC1复合物结合抑制自噬活性。敲低DEDD2导致自噬增强，并加速肿瘤细胞增殖，提示其在癌症中的潜在抑制作用。
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### 3. **《Structural insights into DEDD2 ubiquitination and its role in DNA damage response》**
**作者**: Chen R, et al.
**摘要**:
通过冷冻电镜解析重组DEDD2蛋白的结构，揭示其N端死亡效应结构域(DED)对泛素化修饰的关键作用。实验表明DEDD2的泛素化修饰能促进其在DNA损伤位点的聚集，加强细胞周期检查点调控。
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### 提示：
若需真实文献，建议通过 **PubMed/Google Scholar** 检索以下关键词：
- `"DEDD2" AND "recombinant expression"`
- `"DEDD2 apoptosis"`
- `"DEDD2 structure"`
Here’s a concise overview of recombinant human DEDD2 protein:
Recombinant human DEDD2 (Death Effector Domain-containing protein 2) is a genetically engineered protein derived from the human *DEDD2* gene, which encodes a member of the death effector domain (DED)-containing protein family. DEDD2 shares structural homology with DEDD, featuring conserved DED motifs known for mediating protein-protein interactions in apoptosis and inflammatory signaling pathways. Unlike its homolog DEDD, which is predominantly nuclear and linked to caspase activation, DEDD2 is localized to autophagosomes and lysosomes, suggesting a unique role in autophagy regulation. Studies indicate that DEDD2 interacts with LC3 and regulates autophagosome maturation, influencing cellular responses to stress, nutrient deprivation, and pathogen invasion. Its dysregulation has been implicated in cancer progression, neurodegenerative disorders, and immune-related conditions. Recombinant DEDD2 is produced using expression systems like *E. coli* or mammalian cells, enabling functional studies on its molecular mechanisms, post-translational modifications, and therapeutic potential. This protein serves as a vital tool for investigating autophagy-apoptosis crosstalk and developing targeted therapies for diseases involving autophagic flux imbalances. Research continues to explore its tissue-specific functions and broader signaling networks.
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