| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEF8 |
| Uniprot No | Q6ZN54 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | MAILSLRAPG PWQAMQVWAD RTLLTPHTGV TSQVLGVAAA VMTPLPGGHA AGRTREARWD AMEYDEKLAR FRQAHLNPFN KQSGPRQHEQ GPGEEVPDVT PEEALPELPP GEPEFRCPER VMDLGLSEDH FSRPVGLFLA SDVQQLRQAI EECKQVILEL PEQSEKQKDA VVRLIHLRLK LQELKDPNED EPNIRVLLEH RFYKEKSKSV KQTCDKCNTI IWGLIQTWYT CTGCYYRCHS KCLNLISKPC VSSKVSHQAE YELNICPETG LDSQDYRCAE CRAPISLRGV PSEARQCDYT GQYYCSHCHW NDLAVIPARV VHNWDFEPRK VSRCSMRYLA LMVSRPVLRL REINPLLFSY VEELVEIRKL RQDILLMKPY FITCREAMEA RLLLQLQDRQ HFVENDEMYS VQDLLDVHAG RLGCSLTEIH TLFAKHIKLD CERCQAKGFV CELCREGDVL FPFDSHTSVC ADCSAVFHRD CYYDNSTTCP KCARLSLRKQ SLFQEPGPDV EA |
| 分子量 | 58.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DEF8蛋白的模拟参考文献示例(基于现有知识框架假设，非真实文献，建议通过学术数据库核实最新研究)：
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1. **"Recombinant human DEF8 promotes autophagosome-lysosome fusion via interaction with ESCOC complex"**
*Authors: Chen L, et al.*
**摘要**：本研究通过大肠杆菌系统表达并纯化重组人DEF8蛋白，发现其通过结合ESCOC复合体组分(如VPS37B)调控自噬体成熟过程，为DEF8在神经退行性疾病中的作用提供机制依据。
2. **"Structural and functional analysis of DEF8 in EGFR endosomal sorting"**
*Authors: Wang Y, et al.*
**摘要**：报道了重组DEF8蛋白的晶体结构(分辨率2.8Å)，揭示其UBAP样结构域参与EGFR的ESCRT依赖性降解，验证其通过招募TSG101调控受体酪氨酸激酶的循环。
3. **"DEF8 knockdown enhances viral innate immune signaling via MAVS aggregation"**
*Authors: Singh R, et al.*
**摘要**：利用HEK293T细胞表达重组DEF8蛋白，证明其通过抑制MAVS线粒体聚集体形成负向调控抗病毒反应，提示DEF8在免疫平衡中的双重角色。
4. **"High-yield production of recombinant DEF8 in Pichia pastoris and its role in exosome biogenesis"**
*Authors: Müller S, et al.*
**摘要**：优化毕赤酵母表达系统获得高纯度DEF8蛋白，证明其促进多泡体(MVB)形成及外泌体分泌，为癌症微环境研究提供工具蛋白。
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**注**：DEF8(Differentially Expressed in FDCP 8 homolog)为ESCRT通路相关蛋白，真实研究可能集中于其与膜运输、自噬或免疫调节的关联。建议通过PubMed或Google Scholar以“DEF8 recombinant”或“DEF8 ESCRT”为关键词检索最新文献。
Recombinant human DEF8 (Differentially Expressed in FDCP 8) protein is a genetically engineered version of the naturally occurring DEF8 protein, produced using in vitro expression systems like bacterial, yeast, or mammalian cell cultures. DEF8. a 519-amino-acid protein, is implicated in intracellular trafficking and membrane dynamics. It contains a RUN domain, which mediates interactions with Rab GTPases, and a TBC domain, characteristic of proteins involved in Rab inactivation. Studies suggest DEF8 regulates vesicle transport, lysosomal function, and autophagosome maturation, potentially linking it to autophagy, endocytosis, and immune response pathways. Its role in modulating Rab7 activity highlights its importance in endosomal-lysosomal trafficking.
Recombinant DEF8 is widely used to study these mechanisms in vitro, enabling researchers to investigate protein-protein interactions, enzymatic activities, and regulatory networks in diseases like cancer and neurodegenerative disorders. The recombinant form allows controlled experimental conditions, overcoming challenges of low endogenous expression. Production typically involves cloning the DEF8 cDNA into expression vectors with affinity tags (e.g., His-tag) for purification. Understanding DEF8's molecular functions through recombinant protein studies may contribute to therapeutic strategies targeting membrane trafficking defects. Current research continues to explore its structural features and post-translational modifications, emphasizing its emerging role in cellular homeostasis.
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