| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJC10 |
| Uniprot No | Q8IXB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-793aa |
| 氨基酸序列 | MGVWLNKDDYIRDLKRIILCFLIVYMAILVGTDQDFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNNPNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWNYYRYDFGIYDDDPEIITLERREFDAAVNSGELWFVNFYSPGCSHCHDLAPTWRDFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSGMAPVKYHGDRSKESLVSFAMQHVRSTVTELWTGNFVNSIQTAFAAGIGWLITFCSKGGDCLTSQTRLRLSGMLDGLVNVGWMDCATQDNLCKSLDITTSTTAYFPPGATLNNKEKNSILFLNSLDAKEIYLEVIHNLPDFELLSANTLEDRLAHHRWLLFFHFGKNENSNDPELKKLKTLLKNDHIQVGRFDCSSAPDICSNLYVFQPSLAVFKGQGTKEYEIHHGKKILYDILAFAKESVNSHVTTLGPQNFPANDKEPWLVDFFAPWCPPCRALLPELRRASNLLYGQLKFGTLDCTVHEGLCNMYNIQAYPTTVVFNQSNIHEYEGHHSAEQILEFIEDLMNPSVVSLTPTTFNELVTQRKHNEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSIDCQQYHSFCAQENVQRYPEIRFFPPKSNKAYHYHSYNGWNRDAYSLRIWGLGFLPQVSTDLTPQTFSEKVLQGKNHWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL |
| 分子量 | 117.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJC10蛋白的3-4篇参考文献的简略示例(注：以下内容为模拟生成，可能需要根据实际文献调整)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human DNAJC10 for Structural Studies"*
**作者**：Li, X. et al.
**摘要**：该研究描述了一种高效表达和纯化重组人DNAJC10蛋白的方法，利用大肠杆菌表达系统，并通过质谱和圆二色谱验证其正确折叠。此方法为后续研究DNAJC10的分子机制提供了实验基础。
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2. **文献名称**：*"DNAJC10 Acts as a Molecular Chaperone in ER Stress Response by Regulating Protein Folding"*
**作者**：Wang, Y. & Zhang, H.
**摘要**：本文揭示DNAJC10在内质网应激反应中的作用，证明其通过与错误折叠蛋白结合并协助HSP70伴侣系统，维持细胞稳态，可能成为治疗蛋白质错误折叠相关疾病的靶点。
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3. **文献名称**：*"Role of DNAJC10 in Alzheimer’s Disease: Implications for Tau Protein Aggregation"*
**作者**：Johnson, R. et al.
**摘要**：研究发现DNAJC10在阿尔茨海默病模型细胞中抑制Tau蛋白聚集，提示其通过调节分子伴侣网络减轻神经退行性病变，为开发神经保护策略提供了新方向。
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4. **文献名称**：*"DNAJC10 Overexpression Confers Chemotherapy Resistance in Cancer Cells"*
**作者**：Chen, L. et al.
**摘要**：该论文显示DNAJC10在多种癌症细胞系中高表达，并通过调控抗凋亡蛋白稳定性增加化疗耐药性，提示靶向DNAJC10可能增强化疗敏感性。
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如需真实文献，建议在PubMed或Google Scholar搜索关键词 **"recombinant human DNAJC10"** 或 **"DNAJC10 protein function"** 获取近期研究。
Recombinant human DNAJC10 protein, also known as ERdj5. is a member of the Hsp40/DnaJ protein family, which functions as a co-chaperone for Hsp70 proteins in regulating proteostasis. Primarily localized in the endoplasmic reticulum (ER), DNAJC10 plays a critical role in managing ER stress by facilitating the degradation of misfolded proteins through the ER-associated degradation (ERAD) pathway. It contains thioredoxin-like domains with disulfide reductase activity, enabling it to reduce disulfide bonds in misfolded glycoproteins, thereby preparing them for retrotranslocation to the cytoplasm and subsequent proteasomal degradation. This activity links DNAJC10 to cellular quality control mechanisms and adaptive responses during unfolded protein response (UPR). Dysregulation of DNAJC10 has been implicated in diseases involving ER stress, including neurodegenerative disorders, diabetes, and cancer. Recombinant versions of DNAJC10 are produced using engineered expression systems (e.g., mammalian or bacterial cells) to study its structural, biochemical, and functional properties. These recombinant proteins serve as essential tools for elucidating ER stress pathways, screening therapeutic compounds targeting protein misfolding diseases, and understanding molecular mechanisms of redox regulation in proteostasis. Ongoing research explores its potential as a biomarker or therapeutic target for ER stress-related conditions.
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