| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJC18 |
| Uniprot No | Q9H819 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MAATLGSGERWTEAYIDAVRRNKYPEDTPPESHDPCGCCNCMKAQKEKKSENEWTQTRQGEGNSTYSEEQLLGVQRIKKCRNYYEILGVSRDASDEELKKAYRKLALKFHPDKNCAPGATDAFKAIGNAFAVLSNPDKRLRYDEYGDEQVTFTAPRARPYNYYRDFEADITPEELFNVFFGGHFPTGNIHMFSNVTDDTYYYRRRHRHERTQTQKEEEEEKPQTTYSAFIQLLPVLVIVIISVITQLLATNPPYSLFYKSTLGYTISRETQNLQVPYFVDKNFDKAYRGASLHDLEKTIEKDYIDYIQTSCWKEKQQKSELTNLAGLYRDERLKQKAESLKLENCEKLSKLIGLRRGG |
| 分子量 | 68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJC18蛋白的3篇参考文献示例(注：具体文献需通过数据库验证，以下为模拟概括)：
1. **标题**：*DNAJC18 regulates mitochondrial respiration through interaction with the TIM23 complex*
**作者**：Li X, et al.
**摘要**：研究报道了重组人DNAJC18蛋白在线粒体蛋白转运中的作用，通过结合TIM23复合体调控呼吸链组装，为线粒体功能障碍相关疾病提供新机制。
2. **标题**：*Expression and purification of recombinant human DNAJC18 in Escherichia coli for structural studies*
**作者**：Zhang Y, et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于生产可溶性重组人DNAJC18蛋白，并通过质谱和圆二色光谱验证其结构与功能完整性。
3. **标题**：*DNAJC18 interacts with HSP70 to modulate ER stress response in cancer cells*
**作者**：Wang H, et al.
**摘要**：发现重组人DNAJC18与HSP70协同作用，通过调控内质网应激信号通路影响癌细胞存活，提示其在肿瘤治疗中的潜在靶点价值。
提示：实际文献需通过PubMed/Google Scholar检索确认。DNAJC18研究较少，建议扩展搜索其所属的J蛋白家族(如DNAJB/DNAJC)或结合“Hsp40”等关键词。
The human DNAJC18 protein is a member of the DNAJ/Hsp40 family of molecular chaperones, which play critical roles in regulating Hsp70 ATPase activity and facilitating protein folding, quality control, and cellular stress responses. DNAJC18 is characterized by a conserved N-terminal J-domain responsible for Hsp70 interaction and putative cysteine-rich regions that may mediate redox-sensitive functions. While less studied compared to other DNAJ proteins, emerging evidence suggests its involvement in endoplasmic reticulum (ER)-associated processes, including ER stress adaptation and proteostasis. Recombinant DNAJC18 protein is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) with affinity tags for purification, enabling functional studies and structural characterization. Its recombinant form serves as a tool to investigate molecular mechanisms in protein misfolding diseases, cancer, and metabolic disorders linked to ER dysfunction. Current research focuses on mapping its interactome, substrate specificity, and potential roles in stress signaling pathways. The protein's redox-sensitive domains also hint at involvement in oxidative stress regulation, expanding its relevance to neurodegenerative and cardiovascular conditions. Studies using recombinant DNAJC18 aim to clarify its diagnostic or therapeutic potential as a biomarker or target for pathologies associated with chaperone network imbalances.
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