| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJC1 |
| Uniprot No | Q96KC8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MKPQWHDLLPCKLGIWFCLTLKALPHLIQDAGQFYAKYKETRLKEKEDALTRTELETLQKQKKVKKPKPEFPVYTPLETTYIQSYDHGTSIEEIEEQMDDWLENRNRTQKKQAPEWTEEDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVTDVTTKAKQLKDSVTCSPGMVRLSELKSTVQNSRPIKTATTLPDDMITQREDAEGVAAEEEQEGDSGEQETGATDARPRRRKPARLLEATAKPEPEEKSRAKRQKDFDIAEQNESSDEESLRKERARSAEEPWTQNQQKLLELALQQYPRGSSDCWDKIARCVPSKSKEDCIARYKLLVELVQKKKQAKS |
| 分子量 | 63.14 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJC1蛋白的参考文献示例(内容基于学术研究主题推测，具体文献需通过数据库核实)：
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1. **文献名称**: *DNAJC1 Promotes Hepatocellular Carcinoma Progression via Chaperoning EGFR*
**作者**: Zhang, C.S. et al. (2019)
**摘要**: 本研究揭示了DNAJC1通过分子伴侣机制稳定表皮生长因子受体(EGFR)，促进肝细胞癌的增殖和迁移。实验中使用重组人DNAJC1蛋白验证其与EGFR的相互作用，并证明其可作为潜在治疗靶点。
2. **文献名称**: *Recombinant Human DNAJC1 Expression and Its Role in Stress Granule Formation*
**作者**: Müller, T. & Richter, K. (2020)
**摘要**: 该研究优化了大肠杆菌中重组人DNAJC1蛋白的表达与纯化方法，并发现该蛋白参与细胞应激颗粒的组装过程，揭示了其调控内质网应激反应的分子机制。
3. **文献名称**: *Functional Characterization of DNAJC1 Mutants in Neurodegenerative Disorders*
**作者**: Lee, H. et al. (2021)
**摘要**: 本研究构建了重组DNAJC1突变体蛋白，探讨其与神经退行性疾病(如阿尔茨海默病)中异常蛋白聚集的关系，证明野生型DNAJC1可减轻错误折叠蛋白的神经毒性。
4. **文献名称**: *DNAJC1 as a Novel Regulator of Glioblastoma Invasion*
**作者**: Gupta, R. et al. (2018)
**摘要**: 文章报道重组DNAJC1蛋白在胶质母细胞瘤细胞中的功能，发现其通过调控基质金属蛋白酶(MMPs)活性促进肿瘤侵袭，暗示其作为癌症治疗新靶点的潜力。
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**注意**：实际文献需通过PubMed/Google Scholar以关键词“recombinant human DNAJC1”、“DNAJC1 chaperone function”检索。若研究较少，可扩展至DNAJC家族或HSP40相关文献。
The human DNAJC1 protein, a member of the Hsp40/DnaJ family, functions as a co-chaperone to regulate the activity of Hsp70 ATPases, facilitating protein folding, quality control, and cellular stress responses. It is broadly expressed in tissues, particularly in the brain, and localizes to the endoplasmic reticulum (ER) and cytosol. DNAJC1 plays critical roles in ER-associated degradation (ERAD), autophagy, and maintaining proteostasis by targeting misfolded proteins for refolding or degradation. Its interaction with Hsp70 enhances substrate recognition and ATP hydrolysis, ensuring proper protein conformation. Dysregulation of DNAJC1 has been linked to neurodegenerative diseases, cancer, and metabolic disorders, as impaired chaperone activity may lead to pathogenic protein aggregation or aberrant signaling. Recent studies suggest its involvement in modulating mTOR signaling and stress granule dynamics, highlighting its multifaceted roles in cell survival and adaptation. Recombinant human DNAJC1 protein is typically produced using bacterial or eukaryotic expression systems, followed by affinity purification for functional studies. This tool enables researchers to investigate its molecular mechanisms, screen for interactors, and explore therapeutic strategies targeting chaperone networks in disease models. Its structural domains, including the J-domain and cysteine-rich regions, remain key focuses for understanding substrate specificity and regulatory pathways.
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