| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUB3 |
| Uniprot No | Q6R6M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLPEK SPLSSEARVD LCDDLAPVAR QLAPRKKLPL SSRRPAAVGA GLQNMGNTCY ENASLQCLTY TPPLANYMLS REHSQTCQRP KCCMLCTMQA HITWALHSPG HVIQPSQALA AGFHRGKQED AHEFLMFTVD AMKKACLPGH KQVDHHSKDT TLIHQIFGGC WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVKQALEQL VKPEELNGEN AYHCGLCLQR APASKTLTLH TSAKVLILVL KRFSDVTGNK LAKNVQYPEC LDMQPYMSQQ NTGPLVYVLY AVLVHAGWSC HDGHYFSYVK AQEGQWYKMD DAKVTACSIT SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD RRATQGELKR DHPCLQAPEL DERLVERATQ ESTLDHWKFP QEQNKTKPEF NVRKVEGTLP PNVLVIHQSK YKCGMKNHHP EQQSSLLNLS STTRTDQESV NTGTLASLQG RTRRSKGKNK HSKRALLVCQ |
| 分子量 | 59.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DUB3蛋白的3篇示例参考文献，涵盖不同研究方向：
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1. **文献名称**：*Recombinant human DUB3 modulates cell cycle progression through deubiquitination of CDC25A*
**作者**：Li et al.
**摘要**：该研究利用HEK293细胞表达重组人DUB3蛋白，发现其通过去泛素化并稳定细胞周期蛋白CDC25A，促进G1/S期转换，揭示了DUB3在细胞周期调控中的关键作用。
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2. **文献名称**：*Structural and functional analysis of recombinant human DUB3 in the DNA damage response*
**作者**：Zhang et al.
**摘要**：通过X射线晶体学解析重组DUB3的三维结构，结合体外酶活实验，证明其与BRCA1相互作用并去泛素化，增强DNA损伤修复能力，为靶向DUB3的癌症治疗提供结构基础。
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3. **文献名称**：*Recombinant DUB3 attenuates NF-κB signaling by deubiquitinating TRAF6 in inflammatory models*
**作者**：Wang et al.
**摘要**：研究显示，重组人DUB3蛋白通过去泛素化TRAF6负调控NF-κB通路，抑制炎症因子释放，提示DUB3在炎症性疾病中的潜在治疗价值。
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**注**：以上文献为示例，实际引用时需根据具体研究内容检索真实发表的论文。
Recombinant human DUB3 (deubiquitinating enzyme 3), also known as USP36. is a member of the ubiquitin-specific protease (USP) family that plays critical roles in regulating cellular processes by reversing protein ubiquitination. As a deubiquitinase, DUB3 specifically cleaves ubiquitin-protein bonds, stabilizing substrate proteins involved in cell cycle progression, DNA repair, and apoptosis. It gained attention for its interaction with key regulatory proteins, including the oncogene CDC25A and RhoGDIα, influencing mitotic entry and cell migration. Studies highlight its dual role in cancer: while it exhibits tumor-promoting activity by stabilizing oncoproteins like BRCA1 and CHK1 in some contexts, it may also act as a tumor suppressor in others. Dysregulation of DUB3 has been implicated in malignancies, inflammatory responses, and viral infections. Recombinant DUB3 protein, typically expressed in bacterial or mammalian systems, serves as a vital tool for biochemical assays, structural studies, and inhibitor screening to explore therapeutic strategies. Recent research focuses on resolving its substrate specificity, regulatory mechanisms, and context-dependent functions, as well as its potential as a biomarker or drug target. However, challenges remain in understanding its tissue-specific effects and the complexity of ubiquitination networks it modulates.
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