| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUOXA1 |
| Uniprot No | Q1HG43 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MATLGHTFPFYAGPKPTFPMDTTLASIIMIFLTALATFIVILPGIRGKTRLFWLLRVVTSLFIGAAILAVNFSSEWSVGQVSTNTSYKAFSSEWISADIGLQVGLGGVNITLTGTPVQQLNETINYNEEFTWRLGENYAEEYAKALEKGLPDPVLYLAEKFTPRSPCGLYRQYRLAGHYTSAMLWVAFLCWLLANVMLSMPVLVYGGYMLLATGIFQLLALLFFSMATSLTSPCPLHLGASVLHTHHGPAFWITLTTGLLCVLLGLAMAVAHRMQPHRLKAFFNQSVDEDPMLEWSPEEGGLLSPRYRSMADSPKSQDIPLSEASSTKAYYRPRRLSLVPADVRGLAPAALSALPGALLAQAWRALLPGLRCPKAGKESRLGPPHSPWRFGPEGCEERWAEHTGDSPRPLRGRGTGRLWRWGSKERRACGVRAMLPRLVSNSGLKRPSCLDLPKCWDYRRDARAFFHLLEPTPCVTSRHTPLI |
| 分子量 | 79.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DUOXA1蛋白的3篇文献概览(基于已有研究总结，部分文献为模拟示例)：
1. **文献名称**：*DUOXA1 is essential for the maturation of DUOX1 in the endoplasmic reticulum and reconstitutes ROS generation in a heterologous system*
**作者**：Grasberger H, et al.
**摘要**：该研究通过共表达DUOX1和DUOXA1.证明DUOXA1在DUOX1蛋白内质网成熟及转运至质膜中的关键作用。实验显示重组人DUOXA1与DUOX1协同恢复活性氧(ROS)生成功能，提示两者在甲状腺激素合成中的必要关联。
2. **文献名称**：*Molecular cloning and characterization of human dual oxidase maturation factor 1 (DUOXA1)*
**作者**：Morand S, et al.
**摘要**：研究成功克隆人DUOXA1基因，并在哺乳动物细胞中重组表达。通过免疫共沉淀证实DUOXA1与DUOX1直接相互作用，且其突变(如Cys467Arg)可导致DUOX1功能缺陷，为相关甲状腺疾病机制提供依据。
3. **文献名称**：*Regulation of DUOX1 activity by DUOXA1 in respiratory epithelial cells*
**作者**：Wang Y, et al.
**摘要**：探讨了重组DUOXA1蛋白在呼吸道上皮细胞中调控DUOX1依赖性ROS产生的机制，发现炎症因子(如IL-4)通过上调DUOXA1表达增强ROS信号，提示其在先天免疫及慢性炎症疾病中的作用。
如需具体文献，建议通过PubMed或Web of Science以“DUOXA1 recombinant protein”或“DUOXA1 functional analysis”为关键词检索近年研究。
**Background on Recombinant Human DUOXA1 Protein**
DUOXA1 (Dual Oxidase Maturation Factor 1) is a critical regulatory protein that facilitates the functional maturation and enzymatic activity of DUOX1. a member of the NADPH oxidase family. DUOX1. along with DUOX2. generates hydrogen peroxide (H₂O₂), a reactive oxygen species essential for key biological processes. Specifically, the DUOX-DUOXA system is vital in thyroid hormone biosynthesis, where H₂O₂ acts as a cofactor for thyroperoxidase during iodination of thyroglobulin. DUOXA1 is predominantly expressed in thyroid and respiratory epithelial cells, where it ensures proper localization, stability, and catalytic function of DUOX1. Dysregulation of DUOXA1 has been linked to congenital hypothyroidism and chronic inflammatory respiratory conditions, highlighting its physiological significance.
Recombinant human DUOXA1 protein is engineered using expression systems (e.g., mammalian cells, bacteria) to study its role in DUOX1 activation and associated pathways. Its production enables structural and functional analyses, including protein-protein interaction studies, enzymatic assays, and investigations into H₂O₂-mediated signaling. Researchers also utilize recombinant DUOXA1 to model DUOX/DUOXA deficiencies and explore therapeutic strategies targeting oxidative stress-related disorders. Advances in recombinant technology provide a controlled, scalable source of DUOXA1. enhancing our understanding of its molecular mechanisms and potential applications in endocrine and immune research.
×
