| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUS1L |
| Uniprot No | Q6P1R4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MPKLQGFEFWSRTLRGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEVCPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLLQRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAASWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAVWELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREELAKVKTLEGIAAVSQELKLRCQEEISRQEGAKPTGDLPFHWICQPYIRPGPREGSKEKAGARSKRALEEEEGGTEVLSKNKQKKQLRNPHKTFDPSLKPKYAKCDQCGNPKGNRCVFSLCRGCCKKRASKETADCPGHGLLFKTKLEKSLAWKEAQPELQEPQPAAPGTPGGFSEVMGSALA |
| 分子量 | 79.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟示例(非真实文献，仅供格式参考)：
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1. **文献名称**: "Expression and Characterization of Recombinant Human DUS1L Protein in Escherichia coli"
**作者**: Chen et al. (2020)
**摘要**: 报道了人源DUS1L蛋白在大肠杆菌中的重组表达和纯化，优化了可溶性表达条件，并通过质谱和Western blot验证其正确性，酶活实验证实其磷酸酶活性对MAP激酶通路的调控作用。
2. **文献名称**: "Structural Insights into the Catalytic Mechanism of DUS1L Dual-Specificity Phosphatase"
**作者**: Kumar & Lee (2018)
**摘要**: 通过X射线晶体学解析了DUS1L蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，并证明其特异性识别磷酸化酪氨酸/苏氨酸残基的分子机制。
3. **文献名称**: "DUS1L Regulates Neuronal Apoptosis via p38 MAPK Signaling Pathway"
**作者**: Zhang et al. (2019)
**摘要**: 利用重组DUS1L蛋白进行体外细胞实验，发现其通过去磷酸化p38 MAPK抑制神经元凋亡，提示其在神经退行性疾病中的潜在治疗价值。
4. **文献名称**: "Functional Screening of DUS1L Variants in Cancer Cell Proliferation"
**作者**: Smith et al. (2021)
**摘要**: 评估了重组DUS1L蛋白在肿瘤细胞中的功能，发现其过表达可抑制肺癌细胞增殖，并与EGFR信号通路异常相关。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索关键词(如“DUS1L recombinant human”、“DUS1L phosphatase function”)获取真实文献。
Recombinant human DUS1L protein, also known as dual specificity phosphatase 1-like protein, is a genetically engineered variant of the endogenous DUS1L enzyme involved in cellular signaling regulation. Belonging to the dual-specificity phosphatase (DSP) family, DUS1L is characterized by its ability to dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues on target substrates. It plays a role in modulating key signaling pathways, including MAPK (mitogen-activated protein kinase), which governs processes like cell proliferation, differentiation, and stress response.
The recombinant form is typically produced using expression systems such as E. coli or mammalian cells, enabling high-purity yields for research applications. Its study is motivated by potential links to human diseases; dysregulation of DUS1L has been implicated in cancer progression, neurological disorders, and immune dysfunctions. Researchers utilize recombinant DUS1L to investigate substrate specificity, enzymatic activity, and interactions with signaling partners. Additionally, it serves as a tool for drug discovery, particularly in developing inhibitors or activators targeting phosphatase activity. Structural studies of recombinant DUS1L have also advanced understanding of its catalytic domain and regulatory mechanisms. While its exact physiological roles remain under exploration, DUS1L’s conservation across species underscores its biological importance, making recombinant variants critical for unraveling its function in health and disease.
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