| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DZIP1L |
| Uniprot No | Q8IYY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767aa |
| 氨基酸序列 | MQSPAATAEGLSGPLFGAYTFPTFKFQPRHDSMDWRRISTLDVDRVARELDVATLQENIAGITFCNLDREVCSRCGQPVDPALLKVLRLAQLIIEYLLHCQDCLSASVAQLEARLQTSLGQQQRGQQELGRQADELKGVREESRRRRKMISTLQQLLMQTGTHSYHTCHLCDKTFMNATFLRGHIQRRHAGVAEGGKQKKQEQPVEEVLEELRAKLKWTQGELEAQREAERQRQLQEAELIHQREIEAKKEFDKWKEQEWTKLYGEIDKLKKLFWDEFKNVAKQNSTLEEKLRALQSHSVMESKLGSLRDEESEEWLRQARELQALREKTEIQKTEWKRKVKELHEEHMAEKKELQEENQRLQASLSQDQKKAAAQSQCQISTLRAQLQEQARIIASQEEMIQSLSLRKVEGIHKVPKAVDTEEDSPEEEMEDSQDEQHKVLAALRRNPTLLKHFRPILEDTLEEKLESMGIRKDAKGISIQTLRHLESLLRVQREQKARKFSEFLSLRGKLVKEVTSRAKERQENGAAVSQPDGQPSVKSQQSALVTREAQPKTRTLQVALPSTPAEPPPPTRQSHGSHGSSLTQVSAPAPHPGLHGPSSTPPSSGPGMSTPPFSSEEDSEGDRVQRVSLQPPKVPSRMVPRPKDDWDWSDTETSEENAQPPGQGSGTLVQSMVKNLEKQLEAPAKKPAGGVSLFFMPNAGPQRAATPGRKPQLSEDESDLEISSLEDLPLDLDQREKPKPLSRSKLPEKFGTGPQSSGQPRVPAW |
| 分子量 | 113.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DZIP1L蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**：*DZIP1L regulates ciliary transition zone assembly by interacting with CEP290 in polycystic kidney disease*
**作者**：Smith J, Brown KE, et al.
**摘要**：该研究阐明了DZIP1L通过与CEP290相互作用调控纤毛过渡区组装，发现DZIP1L缺失会导致小鼠肾脏囊肿形成，揭示了其在多囊肾病中的潜在病理机制。
2. **文献名称**：*Recombinant human DZIP1L protein production in HEK293 cells: Purification and functional characterization*
**作者**：Zhang L, Wang Y, et al.
**摘要**：报道了在HEK293细胞中高效表达重组人DZIP1L蛋白的方法，通过亲和层析纯化获得高纯度蛋白，并验证其与DAZ家族蛋白的结合活性。
3. **文献名称**：*DZIP1L is essential for spermatogenesis via binding to DAZAP1 in germ cells*
**作者**：Johnson RB, Tanaka H, et al.
**摘要**：研究发现DZIP1L在精子发生过程中通过结合DAZAP1调控减数分裂，敲除小鼠模型中观察到不育表型，强调了其在生殖生物学中的关键作用。
4. **文献名称**：*Crystal structure of DZIP1L reveals a conserved ciliopathy-related domain*
**作者**：Lee S, Patel R, et al.
**摘要**：首次解析了DZIP1L的晶体结构，发现其N端含保守结构域，可能参与纤毛相关信号通路的蛋白质互作，为疾病突变位点分析提供了结构基础。
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**注意**：以上文献为模拟示例，实际研究请通过PubMed、Google Scholar等平台以“DZIP1L”“recombinant DZIP1L protein”等关键词检索。
DZIP1L (DAZ-interacting protein 1-like) is a vertebrate-specific protein belonging to the DZIP1 family, characterized by a conserved C-terminal DAZ-interacting domain and a zinc-finger motif. It plays critical roles in cilia-related processes, particularly during embryonic development and cellular signaling. Studies link DZIP1L to the regulation of the Hedgehog signaling pathway, essential for tissue patterning and organogenesis, as well as ciliogenesis and spermatogenesis. Its dysfunction has been implicated in congenital disorders, including laterality defects, renal abnormalities, and male infertility.
Recombinant human DZIP1L protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. This engineered protein typically retains functional domains to mimic native interactions, facilitating investigations into its molecular mechanisms. Researchers utilize recombinant DZIP1L to explore its scaffolding properties in signaling complexes, its role in ciliary assembly/disassembly, and its interaction with DAZ-family proteins in germ cell development. Additionally, it serves as a tool for structural studies to resolve 3D conformations and identify therapeutic targets for ciliopathies or reproductive disorders. Current research focuses on clarifying its dual roles in developmental pathways and cilia dynamics, with potential applications in diagnostics and regenerative medicine.
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