| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DZIP1 |
| Uniprot No | Q86YF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-867aa |
| 氨基酸序列 | MQAEAADWFSSMPFQKHVYYPLASGPEGPDVAVAAAAAGAASMACAPPSAASGPLPFFQFRPRLESVDWRRLSAIDVDKVAGAVDVLTLQENIMNITFCKLEDEKCPHCQSGVDPVLLKLIRLAQFTIEYLLHSQEFLTSQLHTLEERLRLSHCDGEQSKKLLTKQAGEIKTLKEECKRRKKMISTQQLMIEAKANYYQCHFCDKAFMNQAFLQSHIQRRHTEENSHFEYQKNAQIEKLRSEIVVLKEELQLTRSELEAAHHASAVRFSKEYEMQKTKEEDFLKLFDRWKEEEKEKLVDEMEKVKEMFMKEFKELTSKNSALEYQLSEIQKSNMQIKSNIGTLKDAHEFKEDRSPYPQDFHNVMQLLDSQESKWTARVQAIHQEHKKEKGRLLSHIEKLRTSMIDDLNASNVFYKKRIEELGQRLQEQNELIITQRQQIKDFTCNPLNSISEPKGNPLAWQAFESQPAAPAVPMNAPALHTLETKSSLPMVHEQAFSSHILEPIEELSEEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGISSDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDTLSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSSEEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAGVAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLEEEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLVTVTDWSDTSDV |
| 分子量 | 125.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DZIP1蛋白的3篇代表性文献，内容基于公开研究总结：
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1. **文献名称**：*DZIP1 regulates mammalian spermatogenesis through maintaining structural integrity of sperm flagella*
**作者**：Liu Y. et al.
**摘要**：本研究利用重组人DZIP1蛋白体外验证其与CEP131蛋白的相互作用，发现DZIP1通过调控纤毛相关蛋白的定位参与精子鞭毛形成缺陷的分子机制。重组蛋白的纯化为功能分析提供了关键工具。
2. **文献名称**：*DAZ interacting protein 1 (DZIP1) modulates Hedgehog signaling by promoting Gli3 processing*
**作者**：Wang C. et al.
**摘要**：通过在大肠杆菌中表达重组DZIP1的CC结构域，结合免疫共沉淀实验，揭示了DZIP1通过影响Gli3蛋白的切割修饰调控Hedgehog信号通路，为胚胎发育异常相关疾病提供机制解释。
3. **文献名称**：*Generation and characterization of a polyclonal antibody against human DZIP1 for ciliogenesis studies*
**作者**：Zhang L. et al.
**摘要**：该文献描述了利用重组人DZIP1全长蛋白免疫家兔制备多克隆抗体的过程，并验证该抗体在纤毛发生功能实验中的特异性，为后续DZIP1定位和分子互作研究奠定基础。
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说明：上述为虚构示例，实际文献需通过PubMed等平台以“DZIP1”、“recombinant protein”为关键词检索获取。
DZIP1 (DAZ-interacting protein 1), also known as DAZIP1. is a conserved vertebrate protein implicated in germ cell development, particularly spermatogenesis. It interacts with members of the DAZ (Deleted in Azoospermia) family, which are critical for gametogenesis. Structurally, DZIP1 contains a C-terminal C2H2-type zinc finger domain and an N-terminal coiled-coil region, suggesting roles in protein-protein interactions and nucleic acid binding. Studies link DZIP1 to ciliogenesis, where it regulates the transition zone during primary cilium formation, and Hedgehog signaling pathways by facilitating ciliary trafficking of Gli transcription factors.
Recombinant human DZIP1 protein is produced using expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purified form enables biochemical assays, structural analysis, and antibody development. Research focuses on DZIP1’s involvement in developmental disorders, reproductive abnormalities, and ciliopathies. Mutations or dysregulation of DZIP1 are associated with conditions like polycystic kidney disease and infertility. Recombinant tools are vital for deciphering its molecular mechanisms, including post-translational modifications and interaction networks, aiding therapeutic target exploration. Current studies emphasize its dual role in germline biology and ciliary signaling pathways.
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