| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERAL1 |
| Uniprot No | O75616 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSALDHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKWTRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPSPAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICANIIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLMDIFLCDVDIRLSVKLLK |
| 分子量 | 73.81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ERAL1蛋白的参考文献摘要(虚构示例，仅供格式参考)：
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1. **Structural basis of GTPase activity in human ERAL1 protein**
*Authors: Smith A, et al.*
摘要：该研究通过X射线晶体学解析了重组人ERAL1蛋白的三维结构，揭示了其GTP结合位点的构象，阐明ERAL1作为线粒体核糖体组装因子的GTP水解机制。
2. **ERAL1 mutations impair mitochondrial RNA metabolism in Perrault syndrome**
*Authors: Brown L, et al.*
摘要：研究构建了重组ERAL1突变体蛋白，发现其在线粒体16S rRNA结合能力缺陷，导致线粒体翻译功能障碍，解释了ERAL1突变引发佩尔综合征的分子基础。
3. **Recombinant ERAL1 regulates apoptosis via caspase-3 activation**
*Authors: Zhang Y, et al.*
摘要：体外实验表明，重组ERAL1蛋白在应激条件下从线粒体释放，通过结合细胞质Apaf-1蛋白激活caspase-3.提示其在线粒体介导的凋亡通路中的作用。
4. **Functional interaction between ERAL1 and mitochondrial ribosome subunits**
*Authors: Tanaka K, et al.*
摘要：利用重组ERAL1蛋白进行体外结合实验，证实其与小核糖体亚基蛋白MRPS22相互作用，为线粒体核糖体组装过程提供机制解释。
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注：以上文献名为示例，实际文献需通过数据库(如PubMed)检索确认。建议用关键词“recombinant human ERAL1”或“ERAL1 protein function”查询最新研究。
Recombinant human ERAL1 (Eukaryotic Ribosomal Assembly Lar1 Homolog 1) protein is a GTPase involved in mitochondrial ribosome biogenesis, particularly in the maturation of the 18S rRNA component of the small ribosomal subunit. ERAL1. also known as Era-like 1. is evolutionarily conserved and shares homology with bacterial Era proteins, which are critical for rRNA processing and ribosome assembly. In humans, ERAL1 functions as a chaperone, binding to and stabilizing nascent mitochondrial 12S rRNA during ribosome subunit assembly, ensuring proper folding and preventing degradation. Its GTPase activity regulates this process, coupling energy hydrolysis to structural rearrangements essential for ribosome maturation.
Dysregulation of ERAL1 has been linked to mitochondrial dysfunction and diseases such as Perrault syndrome and certain cancers. Studies suggest ERAL1's role extends beyond ribosome biogenesis, influencing apoptosis and energy metabolism, making it a potential therapeutic target. Recombinant ERAL1 is typically produced using bacterial or mammalian expression systems, enabling biochemical and structural studies to dissect its molecular mechanisms. Structural analyses reveal conserved GTP-binding domains and rRNA interaction sites, shedding light on its functional versatility. Research on recombinant ERAL1 enhances understanding of mitochondrial translation defects and their implications in human pathology.
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