| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERBB2IP |
| Uniprot No | Q96RT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1272-1371aa |
| 氨基酸序列 | QGHELAKQEIRVRVEKDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFQNTVELIIVREVSS |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ERBB2IP蛋白的3篇参考文献示例：
1. **文献名称**：**Structural Insights into the ERBB2IP Protein via X-ray Crystallography**
**作者**：Smith A, et al.
**摘要**：本研究利用X射线晶体学解析了重组人ERBB2IP蛋白的三维结构，揭示了其富含亮氨酸重复结构域(LRR)的关键构象，并阐明了其与ERBB2/HER2受体胞内结构域的结合模式，为靶向相互作用研究奠定基础。
2. **文献名称**：**ERBB2IP Regulates Ras/MAPK Signaling via Direct Interaction with Shoc2**
**作者**：Borg J, et al.
**摘要**：文章通过重组ERBB2IP蛋白的体外实验，证实其通过与支架蛋白Shoc2结合，负调控Ras/MAPK信号通路，抑制肿瘤细胞增殖，提示其可能的抑癌功能机制。
3. **文献名称**：**Loss of ERBB2IP Expression Promotes Metastasis in HER2-Positive Breast Cancer**
**作者**：Chen L, et al.
**摘要**：研究发现，重组ERBB2IP蛋白的过表达可抑制HER2阳性乳腺癌细胞的迁移和侵袭，临床样本分析显示ERBB2IP低表达与患者不良预后显著相关，提示其作为潜在治疗靶点。
注：上述文献信息及摘要为模拟示例，实际研究需以具体数据库检索结果为准。建议通过PubMed、Google Scholar等平台查询关键词“ERBB2IP recombinant”或“Erbin HER2 interaction”获取真实文献。
Recombinant human ERBB2IP (ERBB2 Interacting Protein), also known as Erbin, is a scaffold protein encoded by the ERBB2IP gene. It belongs to the leucine-rich repeat and PDZ domain (LAP) family, playing critical roles in cell signaling, adhesion, and polarity regulation. ERBB2IP interacts directly with ERBB2/HER2. a receptor tyrosine kinase often overexpressed in cancers like breast cancer, modulating its downstream signaling pathways. Additionally, it binds to proteins such as δ-catenin and E-cadherin, linking membrane receptors to cytoskeletal components and influencing cell-cell junctions. Its involvement in the Hippo pathway and regulation of YAP/TAZ transcriptional activity further highlights its importance in tissue homeostasis and tumor suppression.
Recombinant ERBB2IP protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions, structural domains, and functional mechanisms. This tool aids in exploring its role in cancer progression, neurodevelopment (via interactions with neuronal receptors), and epithelial integrity. Purified recombinant ERBB2IP is utilized in biochemical assays, structural studies, and drug discovery targeting ERBB2-related pathologies. Its production typically includes affinity tags (e.g., His-tag) for efficient isolation, ensuring high purity and activity for experimental reproducibility. Research on this protein contributes to understanding cellular signaling networks and developing therapeutic strategies for ERBB2-driven diseases.
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