| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EYA4 |
| Uniprot No | O95677 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MEDSQDLNEQ SVKKTCTESD VSQSQNSRSM EMQDLASPHT LVGGGDTPGS SKLEKSNLSS TSVTTNGTGG ENMTVLNTAD WLLSCNTPSS ATMSLLAVKT EPLNSSETTA TTGDGALDTF TGSVITSSGY SPRSAHQYSP QLYPSKPYPH ILSTPAAQTM SAYAGQTQYS GMQQPAVYTA YSQTGQPYSL PTYDLGVMLP AIKTESGLSQ TQSPLQSGCL SYSPGFSTPQ PGQTPYSYQM PGSSFAPSST IYANNSVSNS TNFSGSQQDY PSYTAFGQNQ YAQYYSASTY GAYMTSNNTA DGTPSSTSTY QLQESLPGLT NQPGEFDTMQ SPSTPIKDLD ERTCRSSGSK SRGRGRKNNP SPPPDSDLER VFVWDLDETI IVFHSLLTGS YAQKYGKDPP MAVTLGLRME EMIFNLADTH LFFNDLEECD QVHIDDVSSD DNGQDLSTYS FATDGFHAAA SSANLCLPTG VRGGVDWMRK LAFRYRRVKE LYNTYKNNVG GLLGPAKRDA WLQLRAEIEG LTDSWLTNAL KSLSIISTRS NCINVLVTTT QLIPALAKVL LYSLGGAFPI ENIYSATKIG KESCFERIMQ RFGRKVVYVV IGDGVEEEQA AKKHNMPFWR ISSHSDLLAL HQALELEYL |
| 分子量 | 69.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EYA4蛋白的3篇代表性文献(虚构示例，实际需查阅数据库补充)：
1. **"Recombinant Human EYA4: Expression, Purification, and Phosphatase Activity Analysis"**
*作者：Li X, Wang Y, et al.*
摘要：研究报道了人源EYA4蛋白的重组表达及功能验证。通过大肠杆菌系统表达并纯化EYA4.证实其具有酪氨酸磷酸酶活性，并依赖保守的HAD结构域，为后续功能研究奠定基础。
2. **"EYA4 as a Potential Biomarker in Hearing Loss: Structural Insights from Recombinant Protein Studies"**
*作者：Zhang R, et al.*
摘要：通过分析重组EYA4的结构，揭示了其与内耳发育相关蛋白的相互作用，发现特定突变导致磷酸酶活性丧失，可能与遗传性听力障碍相关，提示其作为诊断标记的潜力。
3. **"Oncogenic Role of EYA4 in Colorectal Cancer via DNA Repair Modulation"**
*作者：Chen H, et al.*
摘要：研究利用重组EYA4蛋白，证明其通过去磷酸化H2AX调控DNA损伤修复，促进结直肠癌细胞增殖及化疗抵抗，提示靶向EYA4或为治疗策略。
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**说明**：以上内容为示例，实际文献需通过PubMed或Google Scholar检索关键词“recombinant EYA4”、“EYA4 protein function”等获取。真实研究中，EYA4常涉及听力障碍、癌症进展及DNA修复机制。
The human EYA4 protein, a member of the Eyes Absent (EYA) family, functions as both a transcriptional co-activator and a tyrosine phosphatase, playing critical roles in developmental processes, tissue homeostasis, and disease. EYA4 contains an N-terminal transcriptional activation domain and a C-terminal phosphatase domain, enabling its dual enzymatic and scaffolding functions. It interacts with transcription factors, such as SIX proteins, to regulate gene networks involved in organogenesis, particularly in the inner ear, kidneys, and heart. Mutations in the EYA4 gene are linked to autosomal dominant hearing loss (DFNA10) and cardiovascular abnormalities, while its dysregulation is implicated in cancers, including breast and lung carcinomas, through pathways affecting cell proliferation, apoptosis, and metastasis. Recombinant human EYA4 protein, typically produced via bacterial or mammalian expression systems, is a key tool for studying its structural, biochemical, and functional properties. Researchers use it to investigate EYA4’s role in developmental disorders, tumorigenesis, and signaling cascades like HIPPO and TGF-β. Its purified form also supports drug discovery targeting EYA4-associated pathologies. Studies on recombinant EYA4 have clarified its phosphatase specificity, DNA repair interactions, and post-translational modifications, advancing therapeutic strategies for hearing loss and cancer.
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