| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | F11R |
| Uniprot No | Q9Y624 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV |
| 分子量 | 32.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人F11R蛋白(JAM-A)的参考文献示例，根据研究领域常见方向整理，仅供参考格式和内容框架：
1. **文献名称**：*Recombinant human JAM-A/F11R modulates endothelial barrier function by regulating tight junction dynamics*
**作者**：Bazzoni, G., et al.
**摘要**：该研究通过体外实验表明，重组人F11R蛋白可竞争性抑制白细胞与内皮细胞的粘附，维持内皮屏障完整性，减少炎症因子诱导的渗透性增加。
2. **文献名称**：*Inhibition of platelet aggregation by soluble recombinant F11R/JAM-A: implications for thrombosis therapy*
**作者**：Naik, M.U., et al.
**摘要**：研究发现，重组F11R蛋白能够阻断血小板表面JAM-A与配体的相互作用，显著抑制ADP诱导的血小板聚集，提示其作为抗血栓药物的潜力。
3. **文献名称**：*Targeting tumor-endothelial adhesion with recombinant F11R/JAM-A suppresses metastatic dissemination in vivo*
**作者**：Muller, W.A., et al.
**摘要**：通过动物模型证明，重组F11R蛋白可干扰肿瘤细胞与血管内皮间的JAM-A依赖粘附，有效减少肺癌细胞转移灶的形成。
4. **文献名称**：*Soluble F11R protein attenuates acute lung injury by reducing neutrophil transmigration*
**作者**：Ostermann, G., et al.
**摘要**：在急性肺损伤模型中，重组F11R蛋白通过抑制中性粒细胞跨内皮迁移，显著缓解炎症反应和肺泡损伤，为治疗提供新思路。
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**注意**：以上文献为示例性质，具体作者和标题可能与实际研究存在差异。建议通过学术数据库(如PubMed、Google Scholar)以关键词“recombinant F11R/JAM-A”检索最新文献以获取准确信息。
**Background of Recombinant Human F11R Protein**
The F11 receptor (F11R), also known as Junctional Adhesion Molecule-A (JAM-A), is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays a critical role in maintaining cell-cell adhesion, particularly in endothelial and epithelial barriers, by forming homophilic interactions or heterophilic bonds with integrins. Structurally, F11R consists of two extracellular immunoglobulin-like domains, a single transmembrane region, and a cytoplasmic tail involved in signaling.
F11R is implicated in diverse physiological processes, including platelet activation, leukocyte trafficking, angiogenesis, and immune regulation. Dysregulation of F11R has been linked to pathologies such as inflammation, thrombosis, atherosclerosis, and cancer metastasis. Its interaction with ligands like LFA-1 integrin or viruses (e.g., reovirus) underscores its dual role in homeostasis and disease.
Recombinant human F11R protein is engineered via expression systems (e.g., mammalian or bacterial cells) to produce soluble or membrane-bound forms for research. It enables the study of F11R-mediated signaling, cell adhesion mechanisms, and therapeutic targeting. Applications include screening inhibitors for inflammatory diseases, investigating tumor microenvironments, and developing diagnostics for cardiovascular disorders. The recombinant protein's purity and activity make it a vital tool for dissecting F11R's biological relevance and translational potential.
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