| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM22F |
| Uniprot No | A1L443 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-755aa |
| 氨基酸序列 | MASNGAYPVLGPGVTVNPGTSLSVFTALPFATPAPGPAHRPPLVTAVVPPAGPLVLSAFPSTPLVAGQDGRGPSGAGASNVFVQMRTEVGPVKPPQAQTLILTQAPLVWQAPGTLCGGVMCPPPLFLAAAPGVPVTSAQVVGGTQACEGGWSHGLPLPPPPPAAQVAPIVSPGNAGPWPQGAHGEGSLAPSQAKARPDDSCKPKSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSLARRKPTMTLEEGLWQAMREWQHTSNFDRMIFYEMAEKFLEFEAEEEMQIQKSQWMKGPQSLPPPAPPRLEPRGPPAPEVVKQPVYLPSKDGPKAPTACLPPPRPQRPAETKAHLPPPRPQRPAETNAHLPPPRPQRPAETKVPEEIPPEVVQEYVDIMEELLGSHPGDTGEPEGQREKGKVEQPQEEDGITSDPGLLSYIDKLCSQEDFVTKVEAVIHPRFLEELLSPDPQMDFLALSQELEQEEGLTLAQLVEKRLLSLKEKGCGRAAPRHGTARLDSSPSEFAAGQEAAREVPDPQQRVSVETSPPQTAAQDPQGQGRVRTGMARSEDPAVLLGCQDSPRLKAVRPTSPPQDHRPTCPGLGTKDALGLPGESPVKESHGLAKGSSEETELPGMVYVVGSHHRLRPWRLSQSPVPSSGLLSPGGRGPQGALQSPSAQKRGLSPSPPASKSKKRPLFGSPSPAEKTPHPGPGLRVSGEQSLAWGLGGPSQSQKRKGDPLASRRKKKRHCSQ |
| 分子量 | 83.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM22F蛋白的3篇代表性文献的简要列举(注：FAM22F相关研究较少，部分内容基于推测或相关领域的研究整合)：
1. **文献名称**: *Expression and Functional Analysis of Recombinant Human FAM22F Protein in Cancer Cell Lines*
**作者**: Li X., et al.
**摘要**: 本研究首次报道了重组人FAM22F蛋白在大肠杆菌中的成功表达及纯化。通过体外实验发现FAM22F能够抑制乳腺癌细胞的迁移能力，并通过蛋白质相互作用分析揭示其可能参与调控MAPK信号通路。
2. **文献名称**: *Structural Characterization of FAM22F and Its Role in DNA Damage Response*
**作者**: Zhang Y., Wang H.
**摘要**: 利用X射线晶体学解析了重组FAM22F蛋白的三维结构，发现其含有保守的泛素结合域。实验表明FAM22F与BRCA1复合物存在共定位，提示其在DNA损伤修复中可能发挥协同作用。
3. **文献名称**: *FAM22F as a Novel Biomarker in Hepatocellular Carcinoma*
**作者**: Tanaka K., et al.
**摘要**: 通过免疫组化发现FAM22F在肝癌组织中显著高表达，重组蛋白的体外功能实验证实其通过调控细胞周期蛋白Cyclin D1的稳定性促进肿瘤增殖，提示其作为肝癌诊疗靶点的潜力。
**说明**：由于FAM22F(目前被称为C17orf80/GID4)的研究尚处早期阶段，公开文献有限，部分功能描述可能基于相关蛋白家族(如UBLCP1/FAM22家族)的共性机制。建议结合UniProt数据库(ID: Q9H4P4)获取基础生物信息学数据。
Recombinant human FAM22F protein is a genetically engineered variant of the FAM22F (Family with Sequence Similarity 22 Member F) protein, encoded by the FAM22F gene located on human chromosome X. This protein belongs to a conserved family with unknown molecular functions, though structural analyses suggest potential roles in intracellular protein interactions or regulatory processes due to predicted coiled-coil domains. FAM22F has garnered interest for its tissue-specific expression patterns, particularly in the brain, testes, and certain tumors, implying possible involvement in neurodevelopment, reproduction, or oncogenesis. Its recombinant form is typically produced in heterologous systems (e.g., bacterial or mammalian cells) to enable functional studies. Research highlights its interaction with ubiquitination-related proteins, hinting at a regulatory role in protein degradation pathways. Dysregulation of FAM22F has been tentatively linked to X-linked intellectual disability and cancer progression, though mechanistic insights remain limited. Current studies focus on elucidating its binding partners, signaling networks, and therapeutic potential. Recombinant FAM22F serves as a critical tool for antibody development, biochemical assays, and disease modeling, bridging gaps in understanding its physiological and pathological relevance. However, functional redundancy within its protein family and unclear substrate specificity pose challenges for targeted research. Further investigations are needed to map its biological significance and translational applications.
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