| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM29A |
| Uniprot No | Q7Z4H7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-955aa |
| 氨基酸序列 | MSSASVTAFEKEHLWMYLQALGFEPGPATIACGKIVSHTHLGVNMFDKLNRDAFHIISYFLFQVLDQSLTKEVFKFCWPPFDQKSDTEFRKHCCEWIKRISGECGSSFPQVVGSLFLSPGGPKFIHLMYHFARFVAMKYIKSNSKNSSHHFVETFNIKPQDLHKCIARCHFARSRFLQILQRQDCVTQKYQENAQLSVKQVRNLRSECIGLENQIKKMEPYDDHSNMEEKIQKVRSLWASVNETLMFLEKEREVVSSVLSLVNQYALDGTNVAINIPRLLLDKIEKQMFQLHIGNVYEAGKLNLLTVIQLLNEVLKVMKYERCQADQARLTVDLHYLEKETKFQKERLSDLKHMRYRIKDDLTTIRHSVVEKQGEWHKKWKEFLGLSPFSLIKGWTPSVDLLPPMSPLSFDPASEEVYAKSILCQYPASLPDAHKQHNQENGCRGDSDTLGALHDLANSPASFLSQSVSSSDRNSVTVLEKDTKMGTPKEKNEAISKKIPEFEVENSPLSDVAKNTESSAFGGSLPAKKSDPFQKEQDHLVEEVARAVLSDSPQLSEGKEIKLEELIDSLGSNPFLTRNQIPRTPENLITEIRSSWRKAIEMEENRTKEPIQMDAEHREVLPESLPVLHNQREFSMADFLLETTVSDFGQSHLTEEKVISDCECVPQKHVLTSHIDEPPTQNQSDLLNKKVICKQDLECLAFTKLSETSRMETFSPAVGNRIDVMGGSEEEFMKILDHLEVSCNKPSTNKTMLWNSFQISSGISSKSFKDNDFGILHETLPEEVGHLSFNSSSSSEANFKLEPNSPMHGGTLLEDVVGGRQTTPESDFNLQALRSRYEALKKSLSKKREESYLSNSQTPERHKPELSPTPQNVQTDDTLNFLDTCDLHTEHIKPSLRTSIGERKRSLSPLIKFSPVEQRLRTTIACSLGELPNLKEEDILNKSLDAKEPPSDLTR |
| 分子量 | 135 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人FAM29A蛋白的模拟参考文献示例(注：文献为模拟构造，实际研究中请通过PubMed或SciHub等平台查询真实文献)：
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1. **文献名称**: *FAM29A interacts with Plk1 and regulates mitotic progression*
**作者**: Smith J, et al.
**摘要**: 研究发现重组人FAM29A蛋白通过与有丝分裂关键激酶Plk1相互作用，调控纺锤体形成和细胞周期进展，缺失FAM29A导致染色体分离异常。
2. **文献名称**: *Structural characterization of human FAM29A and its role in DNA damage response*
**作者**: Li Y, et al.
**摘要**: 解析了FAM29A蛋白的结构域特征，并发现其在DNA损伤修复通路中通过与RAD51结合，促进同源重组修复，暗示其潜在肿瘤治疗靶点价值。
3. **文献名称**: *FAM29A overexpression promotes tumorigenesis in breast cancer models*
**作者**: Garcia R, et al.
**摘要**: 实验表明，重组FAM29A蛋白过表达通过激活Wnt/β-catenin信号通路，显著增强乳腺癌细胞增殖、迁移和体内成瘤能力。
4. **文献名称**: *FAM29A: A novel regulator of microtubule dynamics*
**作者**: Chen L, et al.
**摘要**: 通过体外微管结合实验，证实FAM29A直接影响微管稳定性，其功能缺失导致细胞分裂延迟，提示其在细胞骨架调控中的新作用。
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**备注**：上述文献为示例，实际研究中FAM29A的相关文献可能较少，建议通过**UniProt数据库**(查询ID：Q9NYQ3)或**PubMed**检索最新进展。
FAM29A (Family with sequence similarity 29 member A), also known as CKAP2L, is a human protein implicated in regulating microtubule dynamics and mitotic processes. It was initially identified through genomic studies due to its homology with other cytoskeleton-associated proteins. FAM29A interacts with components of the γ-tubulin ring complex (γ-TuRC) and is believed to facilitate spindle formation by promoting microtubule bundling and stabilization during mitosis. Its expression is tightly regulated across the cell cycle, peaking during the G2/M phase, and dysregulation has been linked to chromosomal instability and defective cell division.
Structurally, FAM29A is a large, intrinsically disordered protein with low solubility, posing challenges for biochemical characterization. Recombinant FAM29A protein, typically produced in mammalian or insect expression systems, enables functional studies to dissect its role in cytoskeletal organization, mitotic progression, and potential crosstalk with signaling pathways. Aberrant FAM29A expression has been observed in cancers, including hepatocellular carcinoma and glioblastoma, suggesting its involvement in tumorigenesis and metastasis. However, its precise molecular mechanisms and interacting partners remain under investigation, highlighting the importance of recombinant protein tools for elucidating its pathophysiological roles. Current research focuses on exploring FAM29A as a diagnostic biomarker or therapeutic target in oncology.
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