| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM86C1 |
| Uniprot No | Q9NVL1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MAPEENAGSE LLLQSFKRRF LAARALRSFR WQSLEAKLRD SSDSELLRDI LQKHEAVHTE PLDELYEVLV ETLMAKESTQ GHRSYLLTCC IAQKPSCRWS GSCGGWLPAG STSGLLNSTW PLPSATQRCA SCSPPSYAGL GSDGKRKLIM TRNCFPTEST WRWQS |
| 分子量 | 18.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开发表的文献中关于“重组人FAM86C1蛋白”的研究较为有限，以下参考条目为根据相关领域研究的假设性示例(建议进一步通过PubMed或Google Scholar等平台核实)：
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1. **文献名称**：*"Cloning and Functional Characterization of Human FAM86C1 as a Potential Methyltransferase"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功克隆并表达了重组人FAM86C1蛋白，证实其在体外具有甲基转移酶活性，可能通过修饰组蛋白H3参与表观遗传调控。
2. **文献名称**：*"FAM86C1 Recombinant Protein Suppresses Tumor Growth in Colorectal Cancer Models"*
**作者**：Li H, et al.
**摘要**：通过大肠杆菌表达系统获得高纯度重组人FAM86C1蛋白，动物实验表明其可通过抑制Wnt/β-catenin信号通路降低结直肠癌细胞增殖。
3. **文献名称**：*"Structural Analysis of Human FAM86C1 Reveals a Novel Protein-protein Interaction Domain"*
**作者**：Wang X, et al.
**摘要**：利用X射线晶体学解析了重组FAM86C1蛋白的三维结构，发现其C端存在一个新型相互作用结构域，可能与RNA结合蛋白互作调控mRNA稳定性。
4. **文献名称**：*"FAM86C1 Knockdown and Recombinant Rescue in Neuronal Development"*
**作者**：Chen L, et al.
**摘要**：在神经元细胞中敲低FAM86C1导致突触形成缺陷，而外源添加重组FAM86C1蛋白可部分恢复表型，提示其在神经发育中的潜在作用。
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**注意**：以上内容为模拟参考，实际文献需通过学术数据库验证。若需具体文献，建议结合实验背景进一步检索或联系领域专家。
**Recombinant human FAM86C1 protein** is a member of the FAM86 family, which is part of the 7BS methyltransferase superfamily. Initially identified through genomic analyses, FAM86C1 (also termed METTL25) remains less characterized compared to other methyltransferases but has garnered interest due to its putative role in post-translational modifications. Emerging evidence suggests that FAM86C1 may function as a lysine methyltransferase, potentially targeting histone or non-histone substrates, though its precise biological substrates and activity remain under investigation. Structurally, it contains a conserved S-adenosylmethionine (SAM)-binding domain, characteristic of methyltransferases, which is critical for transferring methyl groups to target molecules.
Studies indicate FAM86C1 may localize to mitochondria or nuclei, linking it to mitochondrial metabolism, epigenetic regulation, or DNA repair pathways. Its dysregulation has been tentatively associated with certain cancers and metabolic disorders, though mechanistic insights are limited. Recombinant FAM86C1 protein is typically produced in *E. coli* or mammalian expression systems to study its enzymatic properties, interaction networks, and roles in cellular processes. Current research focuses on delineating its physiological functions, substrate specificity, and potential therapeutic implications in diseases linked to methylation imbalances. Its study contributes to broader understanding of methyltransferase diversity and their impact on cellular signaling and homeostasis.
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