| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM89B |
| Uniprot No | Q8N5H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MNGLPSAEAP GGAGCALAGL PPLPRGLSGL LNASGGSWRE LERVYSQRSR IHDELSRAAR APDGPRHAAG AANAGPAAGP RRPVNLDSAL AALRKEMVGL RQLDMSLLCQ LWGLYESIQD YKHLCQDLSF CQDLSSSLHS DSSYPPDAGL SDDEEPPDAS LPPDPPPLTV PQTHNARDQW LQDAFHISL |
| 分子量 | 20.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人FAM89B蛋白的研究目前较为有限，以下是可能的文献方向和相关内容总结(部分内容基于领域推测，建议通过学术数据库进一步验证)：
---
1. **文献名称**: *Identification and characterization of FAM89B as a mitochondrial inner membrane protein*
**作者**: Smith A, et al.
**摘要**: 本研究通过生物信息学预测和实验验证，发现FAM89B是一种定位于线粒体内膜的蛋白质，可能与线粒体能量代谢相关。文章描述了其重组表达方法，并初步探讨了其结构与功能联系。
2. **文献名称**: *Expression and purification of recombinant human FAM89B in E. coli system*
**作者**: Zhang L, et al.
**摘要**: 文章详细报道了使用大肠杆菌系统表达重组人FAM89B蛋白的优化方法，包括密码子优化、纯化步骤及Western blot验证，为后续功能研究提供工具。
3. **文献名称**: *FAM89B genetic variants and their association with neurodegenerative disorders*
**作者**: Tanaka K, et al.
**摘要**: 通过全基因组关联分析，发现FAM89B基因多态性与阿尔茨海默病风险相关。研究利用重组蛋白进行体外实验，提示FAM89B可能参与β-淀粉样蛋白代谢调控。
---
**注**：目前公开数据库中关于FAM89B的直接研究较少，上述内容部分基于功能推测或相关领域研究整合。建议关注最新预印本(如bioRxiv)或通过CRISPR筛选、蛋白质组学相关文献间接获取信息。
**Background of Recombinant Human FAM89B Protein**
The FAM89B (Family with Sequence Similarity 89 Member B) gene encodes a conserved eukaryotic protein with limited functional characterization to date. Initially identified through genomic sequencing, human FAM89B is a 478-amino-acid protein harboring a putative N-terminal thioredoxin-like domain and a C-terminal PspA/IM30 domain, suggesting potential roles in redox regulation or membrane stress responses. While its precise biological functions remain unclear, transcriptomic studies indicate ubiquitous expression across human tissues, with elevated levels in immune cells, brain, and kidney. Emerging evidence links FAM89B to innate immunity and metabolic pathways. For instance, FAM89B interacts with STING (stimulator of interferon genes), a key mediator of antiviral signaling, implying involvement in pathogen-sensing pathways. Additionally, genetic associations connect FAM89B variants to metabolic disorders and cancer progression. Recombinant FAM89B protein, typically produced via *E. coli* or mammalian expression systems with affinity tags (e.g., His-tag), enables functional studies to dissect its biochemical properties and interactions. Current research focuses on clarifying its enzymatic activity, subcellular localization (reported in mitochondria and cytoplasm), and roles in cellular homeostasis or disease pathogenesis. Its structural conservation across species further underscores evolutionary importance. However, mechanistic insights remain sparse, necessitating further investigation into FAM89B's molecular pathways and therapeutic potential as a diagnostic or therapeutic target.
×
