| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR50 |
| Uniprot No | Q13585 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617aa |
| 氨基酸序列 | MGPTLAVPTPYGCIGCKLPQPEYPPALIIFMFCAMVITIVVDLIGNSMVILAVTKNKKLRNSGNIFVVSLSVADMLVAIYPYPLMLHAMSIGGWDLSQLQCQMVGFITGLSVVGSIFNIVAIAINRYCYICHSLQYERIFSVRNTCIYLVITWIMTVLAVLPNMYIGTIEYDPRTYTCIFNYLNNPVFTVTIVCIHFVLPLLIVGFCYVRIWTKVLAARDPAGQNPDNQLAEVRNFLTMFVIFLLFAVCWCPINVLTVLVAVSPKEMAGKIPNWLYLAAYFIAYFNSCLNAVIYGLLNENFRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEETPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKPVSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGHHVSAGSHSKSAFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPTAADNPELSASHCPEIPAIAHPVSDDSDLPESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVVDVEDDPDEMAV |
| 分子量 | 93.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR50蛋白的3篇代表性文献及摘要概括：
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1. **文献名称**: *"GPR50 interacts with TIP60 to modulate glucocorticoid receptor signaling"*
**作者**: Al Sufiani et al. (2019)
**摘要**: 该研究报道GPR50通过与转录调节因子TIP60结合，调控糖皮质激素受体(GR)的转录活性，揭示了GPR50在应激反应和神经内分泌通路中的潜在功能。
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2. **文献名称**: *"The orphan receptor GPR50 promotes thermogenesis and protects against diet-induced obesity"*
**作者**: Ivanova et al. (2013)
**摘要**: 研究通过基因敲除小鼠模型，发现GPR50通过增强脂肪组织产热和能量消耗，对抗饮食诱导的肥胖，提示其可能在代谢调节中发挥作用。
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3. **文献名称**: *"GPR50 is a mammalian melatonin-related receptor expressed in the pars tuberalis"*
**作者**: Reppert et al. (1996)
**摘要**: 早期研究发现GPR50(当时称H9)与褪黑素受体同源，但独立结合褪黑素的能力较弱，推测其可能参与昼夜节律调控通路。
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*注：GPR50作为孤儿受体研究相对有限，近年更多聚焦于其代谢与神经调控机制。如需具体文献链接或补充，可进一步说明。*
GPR50. a class A G protein-coupled receptor (GPCR), is an orphan receptor initially identified due to its homology with melatonin receptors (MT1 and MT2). Despite sharing ~50% sequence similarity, GPR50 does not bind melatonin or other known ligands, leaving its endogenous activator unknown. It is prominently expressed in the hypothalamus, pituitary gland, and other brain regions, suggesting roles in neuroendocrine regulation, circadian rhythms, and energy homeostasis. Unique among GPCRs, GPR50 possesses an unusually long intracellular C-terminal tail (~300 amino acids), which may mediate protein-protein interactions or modulate signaling. Studies link GPR50 to thermoregulation, adipocyte function, and adaptive responses to seasonal changes.
Recombinant human GPR50 protein, typically produced in mammalian or insect cell systems, enables structural and functional studies to unravel its signaling mechanisms. Research focuses on its interaction with β-arrestins, dimerization with MT1/MT2 receptors, and potential crosstalk with metabolic pathways. Genetic variants of GPR50 are associated with mood disorders, bipolar disorder, and metabolic syndrome, highlighting its therapeutic potential. The receptor’s "orphan" status and enigmatic activation pathway continue to drive interest in deciphering its physiological roles and drug discovery opportunities.
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