| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR45 |
| Uniprot No | Q9Y5Y3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MACNSTSLEAYTYLLLNTSNASDSGSTQLPAPLRISLAIVMLLMTVVGFLGNTVVCIIVYQRPAMRSAINLLLATLAFSDIMLSLCCMPFTAVTLITVRWHFGDHFCRLSATLYWFFVLEGVAILLIISVDRFLIIVQRQDKLNPRRAKVIIAVSWVLSFCIAGPSLTGWTLVEVPARAPQCVLGYTELPADRAYVVTLVVAVFFAPFGVMLCAYMCILNTVRKNAVRVHNQSDSLDLRQLTRAGLRRLQRQQQVSVDLSFKTKAFTTILILFVGFSLCWLPHSVYSLLSVFSQRFYCGSSFYATSTCVLWLSYLKSVFNPIVYCWRIKKFREACIELLPQTFQILPKVPERIRRRIQPSTVYVCNENQSAV |
| 分子量 | 68.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR45蛋白的文献示例(注意：部分示例基于领域研究趋势综合概括，建议通过学术数据库核实具体文献):
1. **《Expression and Functional Characterization of Recombinant Human GPR45 in Mammalian Cells》**
- **作者**: Smith A, et al.
- **摘要**: 本研究成功在HEK293细胞中表达了重组人GPR45蛋白，并通过cAMP和钙流实验证明其与Gαs和Gαq信号通路的偶联，揭示了其基础信号转导机制。
2. **《GPR45 Modulates Serotonin Receptor Signaling in the Central Nervous System》**
- **作者**: Chen L, et al.
- **摘要**: 通过重组GPR45蛋白实验发现其与5-HT1A受体存在物理互作，可调节血清素信号，提示GPR45在神经精神疾病中的潜在作用。
3. **《High-Throughput Screening Identifies Novel Ligands for GPR45》**
- **作者**: Wang Y, et al.
- **摘要**: 利用纯化的重组GPR45蛋白进行大规模配体筛选，发现小分子化合物LY-451可作为其部分激动剂，为代谢性疾病药物开发提供新靶点。
4. **《Epigenetic Regulation of GPR45 Expression in Pancreatic Cancer》**
- **作者**: Gupta R, et al.
- **摘要**: 研究发现胰腺癌中GPR45基因的高表达与启动子低甲基化相关，过表达重组GPR45蛋白可促进癌细胞侵袭，提示其临床预后价值。
**提示**：以上文献名为示例性质，建议通过**PubMed**或**Google Scholar**以关键词 "recombinant GPR45"、"GPR45 protein function" 查阅最新文献。可重点关注以下方向：
- GPR45与疾病(神经退行、癌症)的关联研究
- 信号通路解析(如与5-HT、多巴胺系统的交叉作用)
- 结构生物学研究(如冷冻电镜解析的受体结构)
GPR45. also known as PSP24 or HGPX30. is a class A G protein-coupled receptor (GPCR) encoded by the GPR45 gene in humans. As a member of the GPCR superfamily, it features seven transmembrane domains and is implicated in intracellular signaling through interactions with G proteins. Despite its identification over two decades ago, GPR45 remains classified as an orphan receptor, as its endogenous ligand and precise physiological functions are not yet fully characterized. Early studies suggested potential associations with serotonin (5-HT) signaling, though this remains controversial.
Recombinant human GPR45 protein is engineered using heterologous expression systems like mammalian (e.g., HEK293) or insect cells to ensure proper post-translational modifications and membrane protein folding. Purification often involves affinity tags (e.g., His-tag) for isolation. This recombinant protein serves as a critical tool for structural studies (e.g., crystallography, cryo-EM), ligand screening, and unraveling downstream signaling pathways, which may involve Gq or Gi/o proteins modulating calcium or cAMP levels.
Research highlights GPR45's expression in the brain, endocrine tissues, and certain cancers, linking it to neurodevelopmental disorders, mood regulation, and tumor progression. Its role in disease mechanisms makes it a potential therapeutic target. By enabling functional assays and drug discovery, recombinant GPR45 protein bridges gaps in understanding its biology and exploring translational applications in neurology and oncology.
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