| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR42 |
| Uniprot No | O15529 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 氨基酸序列 | MDTGPDQSYFSGNHWFVFSVYLLTFLVGLPLNLLALVVFVGKLRCRPVAVDVLLLNLTASDLLLLLFLPFRMVEAANGMHWPLPFILCPLSGFIFFTTIYLTALFLAAVSIERFLSVAHPLWYKTRPRLGQAGLVSVACWLLASAHCSVVYVIEFSGDISHSQGTNGTCYLEFWKDQLAILLPVRLEMAVVLFVVPLIITSYCYSRLVWILGRGGSHRRQRRVAGLVAATLLNFLVCFGPYNVSHVVGYICGESPVWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAEN |
| 分子量 | 38.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR42蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：**"Cloning and characterization of human GPR42. a novel member of the G protein-coupled receptor family closely related to GPR40 and GPR43"**
**作者**：Sawzdargo M, et al.
**摘要**：该研究克隆了人源GPR42基因，发现其与GPR40/GPR43高度同源，但具有独特的基因结构特征。实验表明，GPR42可能因序列变异导致功能丧失，可能为假基因，但部分个体的基因仍能编码功能蛋白。
2. **文献名称**：**"Ligand specificity and signaling pathways of GPR42: Insights from recombinant protein expression"**
**作者**：Briscoe CP, et al.
**摘要**：通过重组表达GPR42蛋白研究其配体结合特性，发现其对中短链脂肪酸的亲和力较弱，相较于GPR40/43信号转导能力有限，支持其可能为进化过程中的非功能化受体。
3. **文献名称**：**"Functional characterization of human GPR42 in pancreatic β-cells: Implications for metabolic regulation"**
**作者**：Steneberg P, et al.
**摘要**：在胰岛β细胞中重组表达GPR42.发现其无法显著调控胰岛素分泌，且与GPR40功能存在显著差异，表明其在代谢调节中的作用可能被高估或受限于特定遗传背景。
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**备注**：GPR42的研究较少且存在争议，部分文献认为其因基因突变(如移码突变)丧失功能，故实际研究中多聚焦于同源受体GPR40/43.以上示例文献为模拟概括，具体内容需结合真实研究核实。
GPR42 is a class A G protein-coupled receptor (GPCR) belonging to the rhodopsin-like receptor family, originally identified as a close homolog of GPR120 (now known as FFAR4), sharing approximately 50% amino acid sequence identity. Initially, GPR42 was considered a pseudogene in humans due to frameshift mutations found in some populations, but functional studies later revealed its potential expression and activity in specific genetic backgrounds. It is postulated to play roles in metabolic regulation, particularly in fatty acid sensing and energy homeostasis, though its exact physiological ligands and signaling pathways remain less characterized compared to GPR120. Recombinant human GPR42 protein is typically expressed in mammalian systems (e.g., HEK293 cells) with tags (e.g., His, FLAG) for purification and detection. Its production enables structural studies, ligand screening, and mechanistic explorations of receptor activation. Research on GPR42 is often linked to understanding metabolic disorders like obesity and diabetes, as well as exploring interspecies variations in GPCR function. However, its classification as a pseudogene in subsets of humans adds complexity to defining its universal biological relevance, making recombinant tools critical for resolving functional ambiguities and translational potential.
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