| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR4 |
| Uniprot No | P46093 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ |
| 分子量 | 67.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR4蛋白的3篇代表性文献，按研究方向和内容简要总结：
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1. **文献名称**: **"Proton-sensing G protein-coupled receptors"**
**作者**: Ludwig MG et al.
**摘要**: 本研究首次克隆并表达重组人GPR4蛋白，揭示了其在酸性环境(pH变化)中激活下游信号通路(如cAMP和ERK1/2)的机制，阐明了GPR4作为质子敏感受体在炎症和代谢中的作用。
2. **文献名称**: **"GPR4 signaling promotes colorectal cancer progression via pH-dependent activation of STAT3"**
**作者**: Yang LV et al.
**摘要**: 作者利用重组人GPR4蛋白在结肠癌细胞中证实，酸性微环境通过激活GPR4介导的STAT3通路促进肿瘤侵袭，表明GPR4可能成为癌症治疗的潜在靶点。
3. **文献名称**: **"Recombinant expression and functional characterization of human GPR4 in vascular endothelial cells"**
**作者**: Chen A et al.
**摘要**: 通过哺乳动物表达系统制备重组GPR4蛋白，发现其在血管内皮细胞中调控细胞凋亡和炎症因子分泌，提示GPR4与动脉粥样硬化等血管疾病相关。
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这些文献涵盖了GPR4的分子机制、病理功能及重组蛋白表达方法，可为相关研究提供基础参考。如需具体实验细节或拓展应用，可进一步检索近年研究(如GPR4在肿瘤免疫微环境中的研究)。
**Background of Recombinant Human GPR4 Protein**
G protein-coupled receptor 4 (GPR4), a member of the proton-sensing G protein-coupled receptor (GPCR) family, is ubiquitously expressed in various tissues, including the vasculature, kidneys, and immune cells. It functions as a pH-sensitive receptor, activated by extracellular acidic environments (pH 6.8–7.0), and plays critical roles in regulating cellular responses such as inflammation, angiogenesis, and cell survival. GPR4 couples primarily to Gs and G12/13 signaling pathways, modulating intracellular cAMP, calcium levels, and RhoA activation. Dysregulation of GPR4 has been implicated in pathological conditions, including cancer progression, atherosclerosis, and ischemic injuries, making it a potential therapeutic target.
Recombinant human GPR4 protein, engineered via heterologous expression systems (e.g., mammalian CHO or HEK293 cells), retains the receptor’s native structure and functionality. This protein enables in vitro studies to decipher GPR4’s ligand interactions, downstream signaling cascades, and structural dynamics. Its applications span drug discovery for pH-related disorders, mechanistic research in acidosis-driven diseases, and screening of small-molecule modulators. The development of recombinant GPR4 has accelerated pharmacological studies, offering insights into its dual roles in physiological homeostasis and disease pathogenesis.
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