| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR63 |
| Uniprot No | Q9BZJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MVFSAVLTAFHTGTSNTTFVVYENTYMNITLPPPFQHPDLSPLLRYSFETMAPTGLSSLTVNSTAVPTTPAAFKSLNLPLQITLSAIMIFILFVSFLGNLVVCLMVYQKAAMRSAINILLASLAFADMLLAVLNMPFALVTILTTRWIFGKFFCRVSAMFFWLFVIEGVAILLIISIDRFLIIVQRQDKLNPYRAKVLIAVSWATSFCVAFPLAVGNPDLQIPSRAPQCVFGYTTNPGYQAYVILISLISFFIPFLVILYSFMGILNTLRHNALRIHSYPEGICLSQASKLGLMSLQRPFQMSIDMGFKTRAFTTILILFAVFIVCWAPFTTYSLVATFSKHFYYQHNFFEISTWLLWLCYLKSALNPLIYYWRIKKFHDACLDMMPKSFKFLPQLPGHTKRRIRPSAVYVCGEHRTVV |
| 分子量 | 47.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人GPR63蛋白的参考文献示例(基于学术背景虚构，供参考)：
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1. **文献名称**：*"Functional characterization of recombinant human GPR63 in neurodevelopment"*
**作者**：Smith A. et al.
**摘要**：本研究通过体外表达重组人GPR63蛋白，发现其激活后促进小鼠神经元突触生长，可能与神经发育相关疾病(如自闭症)存在关联，并揭示其依赖cAMP信号通路发挥作用。
2. **文献名称**：*"Expression and signaling profiling of recombinant GPR63 in HEK293 cells"*
**作者**：Zhang L. et al.
**摘要**：文章建立了重组人GPR63蛋白在HEK293细胞中的稳定表达体系，证实其与Gs蛋白偶联，并通过pH敏感性实验发现其活性受细胞微环境调控，提示潜在生理调节机制。
3. **文献名称**：*"GPR63 interacts with lysosomal proteins and modulates mTOR signaling"*
**作者**：Lee J. et al.
**摘要**：通过重组GPR63蛋白的免疫共沉淀实验，发现其与溶酶体相关蛋白相互作用，可能通过mTOR通路调控细胞代谢与增殖，为肿瘤靶向治疗提供新方向。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed、Web of Science等数据库检索最新实证论文。GPR63作为孤儿受体，其功能研究相对较少，可扩展检索关键词如“GPR63 signaling”或“GPR63 structure”以获取更详细信息。
GPR63. a member of the G protein-coupled receptor (GPCR) family, is an orphan receptor with unclear endogenous ligands. It features seven transmembrane domains and mediates intracellular signaling via G proteins, though its specific coupling mechanisms remain understudied. Predominantly expressed in the brain, kidneys, lungs, liver, and spleen, GPR63 is implicated in neurodevelopment, synaptic plasticity, and cell proliferation. Emerging studies link it to neurodegenerative disorders (e.g., Alzheimer’s), cancer progression, and metabolic dysregulation, though its precise pathological roles require further validation.
Recombinant human GPR63 protein, produced using mammalian or bacterial expression systems, retains structural and functional integrity for in vitro studies. Its production addresses challenges associated with low natural expression levels in tissues. This recombinant tool facilitates ligand identification, receptor activation/deactivation assays, and drug discovery targeting GPCR-related pathways. Additionally, it aids in elucidating GPR63’s interactions with downstream signaling molecules (e.g., cAMP, MAPK) and its potential crosstalk with other receptors. As research expands, recombinant GPR63 is critical for unraveling its physiological significance and therapeutic relevance in human diseases.
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