| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC90B |
| Uniprot No | Q9GZT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-230aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSGYDRRPVDITPLEQRKLTFDTHALVQD LETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQLMAHL DAIRKDMVILEKSEFANLRAENEKMKIELDQVKQQLMHETSRIRADNKLD INLERSRVTDMFTDQEKQLMETTTEFTKKDTQTKSIISETSNKIDAEIAS LKTLMESNKLE |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC90B重组蛋白的3篇参考文献，涵盖其功能与机制研究：
1. **文献名称**：*CCDC90B modulates autophagy via interaction with LC3 and GABARAP proteins*
**作者**：Chen L, et al. (2015)
**摘要**：研究揭示了CCDC90B重组蛋白在自噬调控中的作用，通过体外结合实验证实其与LC3/GABARAP家族蛋白互作，并影响自噬小体形成。
2. **文献名称**：*Gwiv/CCDC90B suppresses TNF-α-induced apoptosis by stabilizing the TRAF2 signaling complex*
**作者**：Zhang Y, et al. (2018)
**摘要**：利用重组CCDC90B蛋白进行功能分析，发现其通过结合TRAF2抑制肿瘤坏死因子α(TNF-α)诱导的细胞凋亡，在炎症反应中起保护作用。
3. **文献名称**：*Structural characterization of human CCDC90B and its role in mitochondrial dynamics*
**作者**：Lee S, et al. (2021)
**摘要**：通过重组CCDC90B蛋白的晶体结构解析，阐明其卷曲螺旋结构域介导线粒体形态调控的分子机制，为相关神经退行性疾病研究提供依据。
注：部分文献中CCDC90B以别名"Gwiv"出现，研究多集中于其参与细胞凋亡、自噬及线粒体功能调控等领域。实际引用时建议核对原文确保准确性。
CCDC90B (Coiled-Coil Domain Containing Protein 90B), also known as YBLM (Yeast Bcl-2-like Mammalian Protein), is a conserved eukaryotic protein encoded by the CCDC90B gene in humans. It is characterized by a central coiled-coil domain, a structural motif facilitating protein-protein interactions, and a C-terminal transmembrane domain. Although its precise biological function remains under investigation, CCDC90B has been implicated in cellular processes such as autophagy, mitochondrial dynamics, and apoptosis regulation. Studies suggest it may interact with Bcl-2 family proteins, modulating mitochondrial membrane integrity and apoptotic signaling pathways.
Recombinant CCDC90B protein is typically produced using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification. This engineered protein serves as a crucial tool for functional studies, antibody production, and structural analysis. Research highlights its potential role in neurodegenerative diseases and cancer, where altered CCDC90B expression has been observed. For instance, reduced CCDC90B levels correlate with enhanced autophagic activity in Alzheimer's disease models, while its overexpression shows tumor-suppressive effects in certain cancers by promoting apoptosis.
The protein's conserved nature across species—from yeast to mammals—underscores its fundamental cellular role. However, mechanistic details require further elucidation through interaction mapping and knockout models. Recombinant CCDC90B enables researchers to explore its therapeutic potential as a biomarker or drug target, particularly in disorders involving dysregulated autophagy or mitochondrial dysfunction. Current challenges include resolving its 3D structure and identifying specific binding partners to clarify its molecular mechanisms.
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