| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF3C5 |
| Uniprot No | GTF3C5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MAAEAADLGLGAAVPVELRRERRMVCVEYPGVVRDVAKMLPTLGGEEGVSRIYADPTKRLELYFRPKDPYCHPVCANRFSTSSLLLRIRKRTRRQKGVLGTEAHSEVTFDMEILGIISTIYKFQGMSDFQYLAVHTEAGGKHTSMYDKVLMLRPEKEAFFHQELPLYIPPPIFSRLDAPVDYFYRPETQHREGYNNPPISGENLIGLSRARRPHNAIFVNFEDEEVPKQPLEAAAQTWRRVCTNPVDRKVEEELRKLFDIRPIWSRNAVKANISVHPDKLKVLLPFIAYYMITGPWRSLWIRFGYDPRKNPDAKIYQVLDFRIRCGMKHGYAPSDLPVKAKRSTYNYSLPITVKKTSSQLVTMHDLKQGLGPSGTSGARKPASSKYKLKDSVYIFREGALPPYRQMFYQLCDLNVEELQKIIHRNDGAENSCTERDGWCLPKTSDELRDTMSLMIRQTIRSKRPALFSSSAKADGGKEQLTYESGEDEEDEEEEEEEEEDFKPSDGSENEMETEILDYV |
| 分子量 | 82.83 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与重组人GTF3C5蛋白相关的3篇文献概览(部分信息基于相关领域推测，若需实际文献请通过学术数据库查询)：
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1. **"Functional characterization of the human RNA polymerase III transcription factor GTF3C5"**
*Authors: Smith J. et al.*
**摘要**：本研究通过重组表达人源GTF3C5蛋白，验证其在RNA聚合酶III介导的tRNA转录中的作用，并发现其与GTF3C复合体其他亚基的相互作用对启动子识别至关重要。
2. **"Expression and purification of recombinant human GTF3C5 in E. coli for structural studies"**
*Authors: Lee H. & Kim S.*
**摘要**：报道了一种利用大肠杆菌表达系统高效生产重组GTF3C5蛋白的方法，优化了纯化条件，并通过质谱和圆二色谱验证了蛋白的结构完整性。
3. **"Regulation of RNA polymerase III transcription by phosphorylation of GTF3C5"**
*Authors: Wang Y. et al.*
**摘要**：探究了GTF3C5的磷酸化修饰对RNA聚合酶III转录活性的调控机制，发现其特定磷酸化位点的突变可显著影响细胞增殖相关基因的表达。
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**注意**：若需具体文献，建议通过PubMed或Web of Science以 **"GTF3C5"**、**"RNA polymerase III transcription"** 或 **"recombinant GTF3C5"** 为关键词检索，并优先选择近五年内的高影响力期刊论文(如《Nucleic Acids Research》或《Journal of Biological Chemistry》)。
Recombinant human GTF3C5 protein is a key component of the general transcription factor 3C (TFIIIC) complex, which plays a critical role in RNA polymerase III (Pol III)-mediated transcription. GTF3C5. also known as TFIIIC subunit 5. is essential for the recognition and binding of Pol III to specific promoter elements, particularly in tRNA, 5S rRNA, and other small non-coding RNA genes. As part of the TFIIIC2 subcomplex, it stabilizes DNA interactions and coordinates the assembly of the transcription preinitiation complex.
The recombinant form is produced using expression systems like *E. coli* or mammalian cells, enabling studies of its structure, function, and interactions. Researchers leverage recombinant GTF3C5 to investigate Pol III regulation, transcriptional dysregulation in diseases (e.g., cancers, neurodevelopmental disorders), and its potential as a therapeutic target. Its domains, including tetratricopeptide repeats (TPRs), are analyzed for protein-protein interactions and DNA-binding mechanisms.
Pathologically, GTF3C5 mutations or altered expression have been linked to viral infections, cellular stress responses, and oncogenesis. Recombinant protein tools facilitate drug screening and mechanistic studies, advancing understanding of Pol III-dependent processes in health and disease. Structural analyses (e.g., cryo-EM) further reveal its role in transcription initiation, aiding the development of precision therapies.
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