| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTPBP1 |
| Uniprot No | O00178 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MDEGCGETIYVIGQGSDGTEYGLSEADMEASYATVKSMAEQIEADVILLRERQEAGGRVRDYLVRKRVGDNDFLEVRVAVVGNVDAGKSTLLGVLTHGELDNGRGFARQKLFRHKHEIESGRTSSVGNDILGFDSEGNVVNKPDSHGGSLEWTKICEKSTKVITFIDLAGHEKYLKTTVFGMTGHLPDFCMLMVGSNAGIVGMTKEHLGLALALNVPVFVVVTKIDMCPANILQETLKLLQRLLKSPGCRKIPVLVQSKDDVIVTASNFSSERMCPIFQISNVTGENLDLLKMFLNLLSPRTSYREEEPAEFQIDDTYSVPGVGTVVSGTTLRGLIKLNDTLLLGPDPLGNFLSIAVKSIHRKRMPVKEVRGGQTASFALKKIKRSSIRKGMVMVSPRLNPQASWEFEAEILVLHHPTTISPRYQAMVHCGSIRQTATILSMDKDCLRTGDKATVHFRFIKTPEYLHIDQRLVFREGRTKAVGTITKLLQTTNNSPMNSKPQQIKMQSTKKGPLTKRDEGGPSGGPAVGAPPPGDEASSVGAGQPAASSNLQPQPKPSSGGRRRGGQRHKVKSQGACVTPASGC |
| 分子量 | 89.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTPBP1蛋白的假设参考文献示例(内容为虚构，仅供示例参考)：
1. **文献名称**：*Structural and functional analysis of recombinant human GTPBP1 reveals its role in GTP hydrolysis*
**作者**：Zhang L, et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组人GTPBP1蛋白，解析了其晶体结构，并证明其通过保守结构域结合GTP，参与调控细胞内GTP酶活性，为研究其分子机制提供了依据。
2. **文献名称**：*GTPBP1 regulates neuronal development via modulating mRNA translation*
**作者**：Kim S, et al.
**摘要**：作者利用HEK293细胞表达重组人GTPBP1.发现其通过与核糖体互作调控特定mRNA的翻译效率，影响神经元突触形成，揭示了GTPBP1在神经发育中的关键作用。
3. **文献名称**：*Recombinant GTPBP1 promotes cancer cell proliferation by activating ERK signaling*
**作者**：Wang Y, et al.
**摘要**：研究通过哺乳动物系统表达GTPBP1.发现其过表达通过激活ERK通路促进肝癌细胞增殖，靶向该蛋白可能成为潜在癌症治疗策略。
4. **文献名称**：*High-yield purification and characterization of GTPBP1 using a novel eukaryotic expression system*
**作者**：Müller J, et al.
**摘要**：开发了一种昆虫细胞表达体系，高效制备功能性重组GTPBP1蛋白，并验证其通过GTP结合诱导构象变化的生化特性，为后续药物筛选奠定基础。
注：以上文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
GTPBP1 (GTP-binding protein 1) is a conserved eukaryotic protein belonging to the GTPase superfamily, characterized by its ability to bind and hydrolyze GTP. It shares structural homology with elongation factor-like (EFL) GTPases and is implicated in diverse cellular processes, including translation regulation, ribosome biogenesis, and stress response. Human GTPBP1 is primarily localized in the cytoplasm and nucleoli, suggesting roles in ribosomal RNA processing and protein synthesis. Studies indicate its involvement in resolving stalled translation complexes under stress conditions, possibly acting as a quality control factor during mRNA translation. Dysregulation of GTPBP1 has been linked to neurodegenerative diseases, such as Alzheimer's, and certain cancers, highlighting its cellular importance. Recombinant human GTPBP1 protein is typically produced in bacterial or mammalian expression systems, enabling in vitro studies of its enzymatic activity, structure, and molecular interactions. Its recombinant form facilitates research into GTPase-driven mechanisms, ribosome dynamics, and disease-related pathways, serving as a tool for drug discovery and functional genomics. Structural analyses using recombinant protein have elucidated its GTP-binding domains and conformational changes during catalytic cycles. Ongoing research aims to clarify its precise biological targets and therapeutic potential.
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