| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTPBP4 |
| Uniprot No | Q9BZE4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634aa |
| 氨基酸序列 | MAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYQIHRIRHFYMRKVKFTQQNYHDRLSQILTDFPKLDDIHPFYADLMNILYDKDHYKLALGQINIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTVIKRQKQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLREQLELFQNIRPLFINKPLIVVANKCDVKRIAELSEDDQKIFTDLQSEGFPVIETSTLTEEGVIKVKTEACDRLLAHRVETKMKGNKVNEVLNRLHLAIPTRRDDKERPPFIPEGVVARRKRMETEESRKKRERDLELEMGDDYILDLQKYWDLMNLSEKHDKIPEIWEGHNIADYIDPAIMKKLEELEKEEELRTAAGEYDSVSESEDEEMLEIRQLAKQIREKKKLKILESKEKNTQGPRMPRTAKKVQRTVLEKEMRSLGVDMDDKDDAHYAVQARRSRSITRKRKREDSAPPSSVARSGSCSRTPRDVSGLRDVKMVKKAKTMMKNAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR |
| 分子量 | 100.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GTPBP4蛋白的示例参考文献(以下内容为模拟示例，实际文献请通过学术数据库查询)：
1. **标题**: "GTPBP4 regulates ribosome assembly and cell cycle progression in human cells"
**作者**: Smith J, et al.
**摘要**: 研究发现GTPBP4通过水解GTP调控核糖体大亚基的成熟过程，敲低GTPBP4导致细胞周期停滞在G1期，提示其与细胞增殖相关。
2. **标题**: "Structural insights into the GTPase activity of human GTPBP4 protein"
**作者**: Chen L, Wang H.
**摘要**: 通过X射线晶体学解析了重组人GTPBP4蛋白的GTP结合域结构，揭示了其催化口袋的关键氨基酸残基，为靶向药物设计提供依据。
3. **标题**: "GTPBP4 interacts with oncogenic KRAS to promote tumorigenesis"
**作者**: Garcia R, et al.
**摘要**: 发现GTPBP4在结直肠癌中高表达，与KRAS蛋白直接互作增强MAPK信号通路，重组GTPBP4蛋白体外实验证实其促进癌细胞侵袭。
4. **标题**: "Recombinant expression and purification of human GTPBP4 for functional analysis"
**作者**: Kim S, et al.
**摘要**: 报道了使用大肠杆菌系统高效表达可溶性重组人GTPBP4蛋白的方法，并通过体外GTP酶活性实验验证其生物活性。
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**备注**：以上为虚构示例，实际文献建议通过PubMed、Google Scholar等平台以关键词"GTPBP4"或"recombinant GTPBP4"检索。真实研究多聚焦于其核糖体合成、癌症关联及结构功能等领域。
GTPBP4. also known as GTP-binding protein 4. is a member of the GTPase family involved in diverse cellular processes, including ribosome biogenesis, RNA processing, and translational regulation. This protein is evolutionarily conserved and shares structural homology with other GTPases, featuring characteristic GTP-binding domains that enable its role in molecular signaling and energy-dependent conformational changes. In humans, GTPBP4 is predominantly localized in the nucleolus, where it participates in the maturation of ribosomal RNA (rRNA) and the assembly of ribosomal subunits, critical steps for protein synthesis. Studies suggest its interaction with ribosome assembly factors and small nucleolar RNAs (snoRNAs), highlighting its importance in maintaining ribosome homeostasis.
Recombinant human GTPBP4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for *in vitro* studies. Its recombinant form enables biochemical characterization, structural analysis (e.g., X-ray crystallography or cryo-EM), and screening for potential inhibitors or interactors. Dysregulation of GTPBP4 has been implicated in cancer progression, neurodevelopmental disorders, and cellular stress responses, making it a subject of interest in disease mechanism research. By studying recombinant GTPBP4. researchers aim to decipher its enzymatic activity, GTP hydrolysis kinetics, and role in ribosomopathies or oncogenesis, offering insights for therapeutic targeting in pathologies linked to translational dysregulation.
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