| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTPBP6 |
| Uniprot No | O43824 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403aa |
| 氨基酸序列 | MTRAEWQVAEATALVHTLDGWSVVQTMVVSTKTPDRKLIFGKGNFEHLTEKIRGSPDVTCVFLNVERMAAPTKKELEAAWGVEVFDRFTVVLHIFRCNARTKEARLQVALAEMPLHRSNLKRDVAHLYRGVGSRYIMGSGESFMQLQQRLLREKEAKIRKALDRLRKKRHLLRRQRTRREFPVISVVGYTNCGKTTLIKALTGDAAIQPRDQLFATLDVTAHAGTLPSRMTVLYVDTIGFLSQLPHGLIESFSATLEDVAHSDLILHVRDVSHPEAELQKCSVLSTLRGLQLPAPLLDSMVEVHNKVDLVPGYSPTEPNVVPVSALRGHGLQELKAELDAAVLKATGRQILTLRVRLAGAQLSWLYKEATVQEVDVIPEDGAADVRVIISNSAYGKFRKLFPG |
| 分子量 | 71.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是近年关于重组人GTPBP6蛋白的假设性参考文献示例(实际文献需通过数据库验证):
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1. **《Functional Characterization of Recombinant Human GTPBP6 in Mitochondrial RNA Processing》**
*作者: Chen et al., 2021*
摘要：研究通过在大肠杆菌中表达重组人GTPBP6蛋白，探究其与线粒体RNA加工的关联，证实其通过GTP水解活性调控RNA剪切。
2. **《GTPBP6 Promotes Tumor Metastasis via Modulating Ribosome Biogenesis》**
*作者: Zhang & Wang, 2020*
摘要：利用重组GTPBP6蛋白进行体外实验，揭示其在癌细胞中通过核糖体合成通路促进肿瘤侵袭的分子机制。
3. **《Crystal Structure Analysis of GTPBP6 Reveals Insights into GTP-binding Specificity》**
*作者: Müller et al., 2019*
摘要：解析重组人GTPBP6的晶体结构，阐明其GTP结合域的关键氨基酸残基，为靶向药物设计提供依据。
4. **《Role of GTPBP6 in Cellular Stress Response and Apoptosis Regulation》**
*作者: Li et al., 2022*
摘要：通过重组蛋白过表达实验，证明GTPBP6在氧化应激条件下通过调控凋亡相关基因抑制细胞死亡。
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**注**：以上为假设性文献，实际研究需通过PubMed/Google Scholar等平台检索关键词 **"recombinant human GTPBP6"** 或 **"GTPBP6 function"** 获取。
**Background of Recombinant Human GTPBP6 Protein**
GTPBP6 (GTP-binding protein 6) is a member of the GTPase family, involved in diverse cellular processes such as ribosomal biogenesis, RNA processing, and signal transduction. This protein binds and hydrolyzes GTP, a critical energy source regulating molecular interactions. In humans, GTPBP6 is encoded by a gene located in the pseudoautosomal region (PAR1) of the X and Y chromosomes, making it uniquely inherited in a sex-independent manner. It shares homology with bacterial Obg-like GTPases, suggesting evolutionary conservation in functions related to cellular stress responses or RNA metabolism.
Dysregulation of GTPBP6 has been tentatively linked to neurodevelopmental disorders, including Down syndrome-associated phenotypes, though its precise mechanistic role remains under investigation. Recombinant human GTPBP6 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, bioactive forms for functional studies. This enables researchers to explore its interactions with ribosomes, nucleic acids, or signaling molecules, and to assess its potential role in disease pathways. Its recombinant form is critical for structural studies (e.g., crystallography) and screening therapeutic compounds targeting GTPase activity, offering avenues for translational research in genetic or metabolic disorders.
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