| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HECTD2 |
| Uniprot No | Q5U5R9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MSEAVRVPSP ATPLVVAAPA PEERKGKESE REKLPPIVSA GAGATAGLDR GAKGQISTFS SFISAVSPKK EAAENRSSPA HLVFPNIKNV REPPPICLDV RQKQRTSMDA SSSEMKAPVL PEPILPIQPK TVKDFQEDVE KVKSSGDWKA VHDFYLTTFD SFPELNAAFK KDATASFNTI EDSGINAKFV NAVYDTLLNT PQDVQKTVLK GIINSLLREW KGPRTKDDLR AYFILLQNPQ FNNTSTYVIY AHLLRQIATL VEADHHFLVH WFKKLSQKRF KQLVERLLQF ISLRLFPAKP EEFPPITKCS WWIPSAAKVL ALLNTANNLV HPPLIPYTDF YNSTLDHIDL MEEYHTWQNF GNSHRFSFCQ YPFVISVAAK KIIIQRDSEQ QMINIARQSL VDKVSRRQRP DMNILFLNMK VRRTHLVSDS LDELTRKRAD LKKKLKVTFV GEAGLDMGGL TKEWFLLLIR QIFHPDYGMF TYHKDSHCHW FSSFKCDNYS EFRLVGILMG LAVYNSITLD IRFPPCCYKK LLSPPIIPSD QNIPVGICNV TVDDLCQIMP ELAHGLSELL SHEGNVEEDF YSTFQVFQEE FGIIKSYNLK PGGDKISVTN QNRKEYVQLY TDFLLNKSIY KQFAAFYYGF HSVCASNALM LLRPEEVEIL VCGSPDLDMH ALQRSTQYDG YAKTDLTIKY FWDVVLGFPL DLQKKLLHFT TGSDRVPVGG MADLNFKISK NETSTNCLPV AHTCFNQLCL PPYKSKKDLK QKLIIGISNS EGFGLE |
| 分子量 | 88.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional analysis of the human HECTD2 ubiquitin ligase"**
- Author: Thompson et al.
- 摘要：通过X射线晶体学解析HECTD2蛋白结构，发现其泛素结合域的特殊构象，并验证其在体外泛素化反应中的催化活性。
2. **"HECTD2 promotes hepatocellular carcinoma progression via ubiquitin-mediated degradation of tumor suppressors"**
- Author: Chen et al.
- 摘要：研究揭示HECTD2在肝癌中高表达，通过泛素化降解抑癌蛋白(如p53)促进肿瘤细胞增殖和转移，机制依赖其E3连接酶活性。
3. **"HECTD2 regulates neurodevelopment by modulating Wnt/β-catenin signaling"**
- Author: Martinez et al.
- 摘要：在小鼠模型中，HECTD2缺失导致神经管发育缺陷，表明其通过泛素化调控Wnt通路关键蛋白稳定性，影响胚胎神经系统的形成。
4. **"HECTD2 interacts with hepatitis B virus core protein and enhances viral replication"**
- Author: Wang et al.
- 摘要：发现HECTD2与乙肝病毒核心蛋白结合，通过泛素化修饰促进病毒颗粒组装，敲低HECTD2显著抑制病毒复制。
备注：以上文献为虚拟概括，实际研究需查阅具体数据库(如PubMed)。
HECTD2. a member of the HECT-domain E3 ubiquitin ligase family, plays a critical role in regulating protein ubiquitination, a post-translational modification essential for cellular processes such as protein degradation, trafficking, and signaling. Found in humans, HECTD2 contains a conserved C-terminal HECT domain responsible for its catalytic activity, enabling the transfer of ubiquitin molecules to target substrates. While its full biological functions remain under investigation, studies suggest HECTD2 involvement in neurodevelopment, immune response modulation, and cancer progression. For instance, it interacts with substrates like HSPA1A (a heat-shock protein) and may influence autophagy or apoptosis pathways. Recombinant human HECTD2 protein is typically produced via heterologous expression systems (e.g., bacteria, insect, or mammalian cells) to study its biochemical properties, substrate specificity, and structural features in vitro. Its recombinant form enables researchers to explore enzymatic mechanisms, screen inhibitors, or dissect interaction networks linked to diseases such as neurodegenerative disorders or malignancies. Current research focuses on clarifying its physiological roles, pathological implications, and therapeutic potential as an E3 ligase-targeted drug candidate.
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