| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEK3 |
| Uniprot No | P51956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506 aa |
| 活性数据 | MDDYMVLRMI GEGSFGRALL VQHESSNQMF AMKEIRLPKS FSNTQNSRKE AVLLAKMKHP NIVAFKESFE AEGHLYIVME YCDGGDLMQK IKQQKGKLFP EDMILNWFTQ MCLGVNHIHK KRVLHRDIKS KNIFLTQNGK VKLGDFGSAR LLSNPMAFAC TYVGTPYYVP PEIWENLPYN NKSDIWSLGC ILYELCTLKH PFQANSWKNL ILKVCQGCIS PLPSHYSYEL QFLVKQMFKR NPSHRPSATT LLSRGIVARL VQKCLPPEII MEYGEEVLEE IKNSKHNTPR KKTNPSRIRI ALGNEASTVQ EEEQDRKGSH TDLESINENL VESALRRVNR EEKGNKSVHL RKASSPNLHR RQWEKNVPNT ALTALENASI LTSSLTAEDD RGGSVIKYSK NTTRKQWLKE TPDTLLNILK NADLSLAFQT YTIYRPGSEG FLKGPLSEET EASDSVDGGH DSVILDPERL EPGLDEEDTD FEEEDDNPDW VSELKKRAGW QGLCDR |
| 分子量 | 57.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEK3蛋白的模拟参考文献示例(虚构内容，仅供格式参考)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human NEK3 Kinase*
**作者**：Zhang Y, et al.
**摘要**：该研究首次报道了人源NEK3激酶的重组表达与纯化，揭示其在体外具有磷酸化组蛋白H1的酶活性，并验证了其对ATP结合位点的依赖性。
2. **文献名称**：*NEK3 Regulates Cell Cycle Progression through Phosphorylation of CDC25C*
**作者**：Li H, Wang X.
**摘要**：研究发现重组NEK3蛋白通过磷酸化CDC25C参与G2/M期调控，敲低NEK3导致细胞周期停滞，提示其在DNA损伤修复通路中的作用。
3. **文献名称**：*Structural Insights into the Catalytic Domain of Human NEK3 Kinase*
**作者**：Smith JL, et al.
**摘要**：通过X射线晶体学解析了NEK3激酶结构域的3D结构，揭示了其独特的ATP结合口袋构象，为靶向抑制剂设计提供了理论依据。
4. **文献名称**：*NEK3 Interacts with 14-3-3 Proteins in a Phosphorylation-Dependent Manner*
**作者**：Chen R, et al.
**摘要**：利用重组NEK3蛋白进行互作组学分析，发现其磷酸化后与14-3-3蛋白结合，介导胞内信号转导，可能与乳腺癌转移相关。
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注：以上内容为模拟生成，如需真实文献，建议通过PubMed/Google Scholar检索关键词**"human NEK3 protein"**或**"recombinant NEK3 kinase"**获取。
Recombinant human NEK3 (NIMA-related kinase 3) protein is a member of the NEK serine/threonine kinase family, which shares structural homology with the Aspergillus nidulans NIMA kinase involved in cell cycle regulation. NEK3 is encoded by the NEK3 gene located on chromosome 13q14.11 and is widely expressed in human tissues, with high levels observed in the brain, heart, and skeletal muscle. Unlike other NEK kinases, NEK3 is primarily associated with non-mitotic functions, including signal transduction, cytoskeletal reorganization, and DNA damage response. It contains a conserved N-terminal kinase domain and a C-terminal regulatory region, facilitating interactions with proteins such as PIN1. VCP, and STAT3.
Studies suggest NEK3 plays a role in cancer progression, particularly in breast cancer, where it promotes cell survival, migration, and metastasis through AKT and MAPK signaling pathways. It also modulates immune responses by regulating cytokine signaling and has been implicated in viral infection mechanisms, including hepatitis C virus replication. Recombinant NEK3 protein, typically produced in E. coli or mammalian expression systems with tags (e.g., GST, His), enables functional studies of its enzymatic activity, substrate specificity, and regulatory mechanisms. Its recombinant form is essential for exploring therapeutic targeting opportunities in oncology and infectious diseases.
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