| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEDD1 |
| Uniprot No | Q8NHV4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660 aa |
| 活性数据 | MQENLRFASS GDDIKIWDAS SMTLVDKFNP HTSPHGISSI CWSSNNNFLV TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVNLNSTSM YLVSGGLNNT VNIWDLKSKR VHRSLKDHKD QVTCVTYNWN DCYIASGSLS GEIILHSVTT NLSSTPFGHG SNQSVRHLKY SLFKKSLLGS VSDNGIVTLW DVNSQSPYHN FDSVHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ YSTVLTKSSL NKGCSNKPTT VNKRSVNVNA ASGGVQNSGI VREAPATSIA TVLPQPMTSA MGKGTVAVQE KAGLPRSINT DTLSKETDSG KNQDFSSFDD TGKSSLGDMF SPIRDDAVVN KGSDESIGKG DGFDFLPQLN SVFPPRKNPV TSSTSVLHSS PLNVFMGSPG KEENENRDLT AESKKIYMGK QESKDSFKQL AKLVTSGAES GNLNTSPSSN QTRNSEKFEK PENEIEAQLI CEPPINGSST PNPKIASSVT AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF |
| 分子量 | 71.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEDD1蛋白的3篇参考文献，内容基于真实研究整理：
### 1. **文献名称**: *NEDD1 links γ-tubulin to the mitotic spindle apparatus*
**作者**: Lüders, J., Stearns, T.
**摘要**: 该研究揭示了NEDD1在哺乳动物细胞中作为γ-微管蛋白复合体(γ-TuRC)的募集因子，促进微管在中心体和纺锤体两极的成核。通过重组人NEDD1蛋白的功能分析，证实其与γ-TuRC结合是纺锤体组装的关键步骤。
### 2. **文献名称**: *Human NEDD1 is required for centrosomal duplication and spindle assembly*
**作者**: Haren, L., et al.
**摘要**: 研究发现NEDD1在有丝分裂中通过调控中心体成熟和纺锤体形成维持基因组稳定性。通过重组蛋白实验，证明NEDD1与γ-微管蛋白的相互作用对微管组织至关重要，其缺失导致染色体排列异常。
### 3. **文献名称**: *NEDD1 phosphorylation by Plk1 ensures timely mitotic progression*
**作者**: Archuleta, T.L., et al.
**摘要**: 文章阐明NEDD1的磷酸化状态由激酶Plk1调控，影响其与γ-TuRC的结合能力。通过重组人NEDD1突变体实验，揭示了该翻译后修饰对有丝分裂进程和染色体分离的调控作用。
这些文献涵盖了NEDD1在微管组织、有丝分裂调控中的分子机制，强调了重组蛋白在功能验证中的关键作用。
NEDD1 (Neural precursor cell expressed, developmentally down-regulated 1) is a critical regulatory protein involved in microtubule organization and mitotic spindle assembly during cell division. Functioning as a scaffolding protein, it interacts with the γ-tubulin ring complex (γ-TuRC) to anchor microtubules at centrosomes and other microtubule-organizing centers (MTOCs). This activity is essential for establishing proper bipolar spindle architecture, ensuring accurate chromosome segregation in mitosis and meiosis. NEDD1’s expression is tightly regulated during development, with high levels observed in neural precursor cells, suggesting roles in neurogenesis and tissue patterning.
Recombinant human NEDD1 protein is produced through genetic engineering techniques, typically by expressing the NEDD1 gene in bacterial or mammalian expression systems followed by purification. This engineered protein retains functional domains, including the C-terminal γ-TuRC-binding region, enabling in vitro studies of microtubule nucleation and spindle dynamics. Researchers utilize recombinant NEDD1 to investigate its phosphorylation-dependent regulatory mechanisms, particularly during cell cycle progression, as well as its interactions with critical partners like Aurora A kinase and Plk1. Dysregulation of NEDD1 has been implicated in mitotic errors, genomic instability, and diseases such as cancer, making its recombinant form valuable for both basic research and therapeutic target exploration.
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