| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEDD9 |
| Uniprot No | Q14511 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-834 aa |
| 活性数据 | MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPMQETASSHEQPASGLMQQTFGQQKLYQVPNPQAAPRDTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQRSIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKPQGVYDIPPTKGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYNCDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLHNPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQALEMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPGPGSLHLKNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGVSAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQDIRNKVMNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF |
| 分子量 | 119.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEDD9蛋白的参考文献示例(仅供参考，具体文献需通过学术数据库检索)：
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1. **《NEDD9 promotes invasive cell migration in cancer progression》**
- **作者**: Smith A, et al.
- **摘要**: 研究报道重组人NEDD9蛋白通过激活RAC1和FAK信号通路增强癌细胞迁移和侵袭能力，可能成为肿瘤转移的治疗靶点。
2. **《Structural and functional characterization of recombinant human NEDD9》**
- **作者**: Lee JH, et al.
- **摘要**: 利用X射线晶体学解析NEDD9蛋白的三维结构，揭示其SH3和SD结构域在调控细胞黏附和增殖中的分子机制。
3. **《NEDD9 regulates epithelial-mesenchymal transition in glioblastoma》**
- **作者**: Chen L, et al.
- **摘要**: 实验表明重组NEDD9蛋白通过调控EMT相关基因(如Snail和E-cadherin)促进胶质母细胞瘤的恶性表型。
4. **《The role of NEDD9 in TGF-β signaling and tumor microenvironment》**
- **作者**: Patel R, et al.
- **摘要**: 研究发现重组NEDD9蛋白通过与TGF-β受体相互作用，影响肿瘤微环境中基质细胞的活化和转移潜能。
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**建议**：通过PubMed或Web of Science检索关键词(如“recombinant NEDD9”、“NEDD9 protein function”)获取真实文献。注意筛选近年高影响力期刊的研究(如《Oncogene》《Nature Cell Biology》等)。
NEDD9 (Neural precursor cell expressed developmentally downregulated 9), also known as HEF1 or Cas-L, is a scaffolding protein belonging to the Cas (Crk-associated substrate) family. Structurally, it contains multiple protein interaction domains, including an N-terminal Src homology 3 (SH3) domain, a substrate domain with tyrosine phosphorylation sites, and a C-terminal helix-loop-helix motif. Functionally, NEDD9 integrates signals from cell surface receptors (e.g., integrins, growth factor receptors) to regulate diverse cellular processes, including adhesion, migration, invasion, and apoptosis. It acts as a signaling hub in pathways such as Ras/MAPK and PI3K/AKT, modulating cytoskeletal dynamics and extracellular matrix interactions.
This protein has gained significant attention in cancer biology due to its dual roles in tumor progression. Elevated NEDD9 expression correlates with enhanced metastatic potential in melanoma, breast cancer, and glioblastoma by promoting epithelial-mesenchymal transition (EMT) and invadopodia formation. Conversely, it may act as a tumor suppressor in other contexts by inducing senescence. Beyond oncology, NEDD9 participates in neurodevelopment, immune response modulation, and cardiovascular functions. Recombinant human NEDD9 protein (rhNEDD9) is typically produced in E. coli or mammalian expression systems, enabling mechanistic studies of its phosphorylation-dependent interactions and therapeutic targeting. Its context-dependent functionality continues to make it a compelling subject for studying cellular signaling complexity.
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