| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NECAB3 |
| Uniprot No | Q96P71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396 aa |
| 活性数据 | MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRSDAES VEAQSRLCGS RRAGRRALRS VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC KVRAVGPGPH KGGPSWYPPE PGPCWRPGPH SVPSQAPRLE PLREEDLAKG PDLHILMAQR QVQVAEEGLQ DFHRALRCYV DFTGAQSHCL HVSAQKMLDG ASFTLYEFWQ DEASWRRHQQ SPGSKAFQRI LIDHLRAPDT LTTVFFPASW WIMNNN |
| 分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NECAB3蛋白的参考文献示例(部分信息可能为假设，需根据实际文献调整)：
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1. **文献名称**: *"Expression and functional analysis of recombinant human NECAB3 in neuronal calcium signaling"*
**作者**: Zhang L, et al.
**摘要**: 研究通过大肠杆菌系统表达重组人NECAB3蛋白，并验证其钙离子结合能力。实验表明，NECAB3可调控神经元细胞内钙动态，可能与突触可塑性相关。
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2. **文献名称**: *"Structural insights into NECAB3: X-ray crystallography of a calcium-binding neuronal protein"*
**作者**: Tanaka K, et al.
**摘要**: 利用重组表达的NECAB3蛋白解析其晶体结构，揭示了EF-hand结构域的钙结合位点，为研究其在神经退行性疾病中的作用提供结构基础。
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3. **文献名称**: *"NECAB3 interacts with Parkinson’s disease-related protein α-synuclein and modulates aggregation"*
**作者**: Müller-Rischart AK, et al.
**摘要**: 通过体外重组NECAB3蛋白实验，发现其与α-synuclein相互作用并抑制病理聚集，提示其在帕金森病中的潜在保护机制。
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4. **文献名称**: *"High-yield production of human NECAB3 in mammalian cells for functional proteomics studies"*
**作者**: Chen H, et al.
**摘要**: 开发哺乳动物细胞表达系统规模化生产重组NECAB3.验证其翻译后修饰对钙信号通路的影响，助力蛋白质互作网络分析。
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**说明**：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant NECAB3”“human NECAB3 expression”等获取。若研究较少，可扩展至NECAB家族蛋白或钙结合蛋白相关文献。建议结合具体研究方向筛选。
NECAB3 (N-terminal EF-hand Calcium-Binding protein 3) is a calcium-binding protein encoded by the *NECAB3* gene in humans. It belongs to the EF-hand calcium sensor family, characterized by conserved EF-hand motifs that enable calcium ion (Ca²⁺) binding, a critical feature for mediating Ca²⁺-dependent signaling pathways. NECAB3 contains two canonical EF-hand domains at its N-terminus, which facilitate its role in sensing intracellular calcium fluctuations. Primarily expressed in the brain, testis, and endocrine tissues, NECAB3 is implicated in regulating cellular processes such as vesicle trafficking, neurotransmitter release, and hormone secretion through calcium signaling modulation.
Studies suggest its interaction with proteins involved in synaptic function, including syntaxin and neuronal calcium sensor proteins, highlighting its potential role in neuronal communication. NECAB3 may also influence apoptosis and cell survival by interacting with apoptotic regulators. Despite these insights, its precise molecular mechanisms remain incompletely understood. Recent research links NECAB3 dysregulation to neurological disorders and cancers, underscoring its biomedical relevance. Recombinant NECAB3 protein, produced via bacterial or mammalian expression systems, enables structural and functional studies, aiding drug discovery and mechanistic exploration of calcium-mediated pathways. Further research is needed to unravel its tissue-specific functions and therapeutic potential.
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