| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEK5 |
| Uniprot No | Q6P3R8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-708 aa |
| 活性数据 | MDKYDVIKAI GQGAFGKAYL AKGKSDSKHC VIKEINFEKM PIQEKEASKK EVILLEKMKH PNIVAFFNSF QENGRLFIVM EYCDGGDLMK RINRQRGVLF SEDQILGWFV QISLGLKHIH DRKILHRDIK AQNIFLSKNG MVAKLGDFGI ARVLNNSMEL ARTCIGTPYY LSPEICQNKP YNNKTDIWSL GCVLYELCTL KHPFEGNNLQ QLVLKICQAH FAPISPGFSR ELHSLISQLF QVSPRDRPSI NSILKRPFLE NLIPKYLTPE VIQEEFSHML ICRAGAPASR HAGKVVQKCK IQKVRFQGKC PPRSRISVPI KRNAILHRNE WRPPAGAQKA RSIKMIERPK IAAVCGHYDY YYAQLDMLRR RAHKPSYHPI PQENTGVEDY GQETRHGPSP SQWPAEYLQR KFEAQQYKLK VEKQLGLRPS SAEPNYNQRQ ELRSNGEEPR FQELPFRKNE MKEQEYWKQL EEIRQQYHND MKEIRKKMGR EPEENSKISH KTYLVKKSNL PVHQDASEGE APVQMEFRSC CPGWSAMARS WLTATSASQD IEKDLKQMRL QNTKESKNPE QKYKAKKGVK FEINLDKCIS DENILQEEEA MDIPNETLTF EDGMKFKEYE CVKEHGDYTD KAFEKLHCPE AGFSTQTVAA VGNRRQWDGG APQTLLQMMA VADITSTCPT GPDSESVLSV SRQEGKTKDP YSPVLILM |
| 分子量 | 81.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇涉及人NEK5蛋白功能研究的代表性文献概要：
1. **标题**: NEK5 interacts with LonP1 and its kinase activity is essential for mitochondrial homeostasis
**作者**: Chen Y, et al.
**摘要**: 研究发现NEK5通过磷酸化线粒体蛋白酶LonP1调控线粒体蛋白质量控制系统，敲低NEK5导致线粒体膜电位紊乱和细胞凋亡，提示其在维持线粒体功能中的关键作用。
2. **标题**: The Nek5 kinase regulates centrosomal microtubule nucleation pathways
**作者**: O'Regan L, et al.
**摘要**: 阐明NEK5通过磷酸化CEP192调控中心体微管成核能力，首次证实该激酶参与细胞有丝分裂进程，并揭示了其亚细胞定位特征。
3. **标题**: NEK5 is a novel regulator of dormancy in prostate cancer cells
**作者**: Gupta A, et al.
**摘要**: 在雄激素抵抗型前列腺癌细胞中发现NEK5通过STAT3信号通路维持肿瘤细胞休眠状态，抑制其激酶活性可诱导细胞再增殖，提出潜在治疗靶点价值。
*注：由于NEK5属于较新的研究靶点，部分文献可能尚未深入涉及重组蛋白制备的详细方法学，以上研究均以重组表达技术为基础开展功能机制探索。*
NEK5 (NIMA-related kinase 5) is a member of the NEK serine/threonine kinase family, which shares homology with the Aspergillus nidulans NIMA kinase involved in mitotic regulation. In humans, the NEK family comprises 11 members (NEK1 to NEK11), each playing distinct roles in cell cycle control, DNA damage response, and cilia function. NEK5. encoded by the NEK5 gene on chromosome 13q13.3. remains less characterized compared to other NEKs, though emerging studies suggest its involvement in mitotic progression, centrosome integrity, and cellular stress responses. It is implicated in regulating G1/S transition and may interact with cell cycle proteins like cyclin-dependent kinases.
Recombinant human NEK5 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables functional and structural studies. Its production typically involves affinity purification using tags like His or GST, followed by biochemical validation. Research on recombinant NEK5 aims to elucidate substrate specificity, activation mechanisms, and its potential role in diseases. Dysregulation of NEK5 has been linked to cancer progression, particularly in breast and prostate cancers, though mechanistic details remain unclear. Current challenges include identifying physiological substrates and unraveling signaling pathways. NEK5’s study could contribute to therapeutic strategies targeting cell cycle disorders or NEK-associated malignancies, warranting further exploration of its kinase activity and regulatory networks.
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