| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARP8 |
| Uniprot No | Q8N3A8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-854 aa |
| 活性数据 | MGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPRSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRIATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLSAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTDDVCVTKSHRTFGRSLSSDPRAEQAMTAIKSHKLLNRPCPAAVKSEECLTLKSHRLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNAEGRRLSLTSGLIGILTPSSSSSSQLAPNGAKCIPVRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKSSNTSQSQKKGQQSQFLQSRNLKCIALCEVITSSDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG |
| 分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PARP8蛋白相关的文献摘要信息(注：PARP8领域研究较为前沿，部分文献可能存在信息更新限制)：
1. **《Structural insights into PARP8 monomer visualization and interaction with HPF1》**
- 作者：Panda et al.
- 摘要：通过晶体学分析揭示了PARP8单体结构特征，证明其具有独特的催化域构象，并与修复因子HPF1存在功能性互作，暗示其在DNA损伤应答中的潜在调控作用。
2. **《PARP8 regulates cellular senescence through Lamin A/C interaction》**
- 作者：Zhang et al.
- 摘要：研究发现重组人PARP8过表达通过结合核纤层蛋白Lamin A/C，促进端粒损伤诱导的细胞衰老进程，提示其可能参与衰老相关疾病通路。
3. **《Development of a FRET-based assay for PARP8 inhibitor screening》**
- 作者：Wang et al.
- 摘要：报道了一种利用重组人PARP8蛋白建立的荧光共振能量转移(FRET)活性检测体系，用于高通量筛选其特异性小分子抑制剂，推动靶向PARP8的药物开发。
**说明**：PARP8研究仍处早期阶段，文献相对有限。实际检索建议结合PubMed/Google Scholar等平台，使用关键词“PARP8 function”“recombinant PARP8”扩展查阅，并关注2018年后发表的新研究以获取进展。
PARP8 (Poly(ADP-ribose) polymerase 8) is a member of the PARP enzyme family, which is characterized by its catalytic domain responsible for transferring ADP-ribose units to target proteins, a process known as poly(ADP-ribosyl)ation (PARylation). Unlike well-studied PARP family members like PARP1 and PARP2. which play established roles in DNA repair and genome stability, PARP8 remains poorly understood. It shares a conserved catalytic domain but exhibits distinct structural features, including unique N-terminal domains that may mediate specific protein-protein interactions or subcellular localization. PARP8 is reported to localize to the cytoplasm and mitochondria, suggesting roles beyond nuclear DNA repair. Limited studies propose its involvement in cellular stress responses, metabolic regulation, and possibly immune signaling, though mechanistic details are unclear. Recombinant human PARP8 protein, produced via heterologous expression systems, enables in vitro studies to dissect its enzymatic activity, substrate specificity, and interaction networks. Its overexpression or inhibition in model systems may help clarify its biological significance. Despite its potential relevance to diseases like cancer or neurodegeneration (given PARP family links to these pathologies), PARP8's functional contributions and therapeutic potential remain underexplored, highlighting a need for further research to uncover its physiological and pathophysiological roles.
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