| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARVB |
| Uniprot No | Q9HBI1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364 aa |
| 活性数据 | MSSAPRSPTP RPRRMKKDES FLGKLGGTLA RKRRAREVSD LQEEGKNAIN SPMSPALVDV HPEDTQLEEN EERTMIDPTS KEDPKFKELV KVLLDWINDV LVEERIIVKQ LEEDLYDGQV LQKLLEKLAG CKLNVAEVTQ SEIGQKQKLQ TVLEAVHDLL RPRGWALRWS VDSIHGKNLV AILHLLVSLA MHFRAPIRLP EHVTVQVVVV RKREGLLHSS HISEELTTTT EMMMGRFERD AFDTLFDHAP DKLSVVKKSL ITFVNKHLNK LNLEVTELET QFADGVYLVL LMGLLEDYFV PLHHFYLTPE SFDQKVHNVS FAFELMLDGG LKKPKARPED VVNLDLKSTL RVLYNLFTKY KNVE |
| 分子量 | 41.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PARVB蛋白的3篇代表性文献：
1. **《Recombinant human Parvin-β (PARVB) expression and its role in cancer cell adhesion》**
*作者：Zhang Y et al.*
摘要：研究利用大肠杆菌系统表达重组人PARVB蛋白，发现其通过调节整合素信号通路增强肿瘤细胞黏附能力，影响转移潜能。
2. **《Structural characterization of PARVB pleckstrin homology domain and its binding to ILK》**
*作者：Lee S et al.*
摘要：通过杆状病毒系统获得重组PARVB蛋白，解析其PH结构域与整合素连接激酶(ILK)的结合模式，揭示机械信号传导的结构基础。
3. **《PARVB overexpression exacerbates cardiac hypertrophy through Hippo pathway modulation》**
*作者：Chen X et al.*
摘要：利用哺乳动物细胞表达重组PARVB，证实其异常表达通过Hippo-YAP通路调控心肌细胞肥大，为心脏病机制研究提供新靶点。
注：文献检索建议结合PubMed/Google Scholar使用关键词"recombinant PARVB protein"或"PARVB overexpression"获取最新进展，部分前沿研究可能未正式发表。
Parvin beta (PARVB), a member of the parvin protein family, plays a critical role in cell adhesion, migration, and signaling. It is one of three parvins (α, β, and γ) characterized by their ability to interact with integrin-linked kinase (ILK), a key component of cell-matrix adhesion complexes. PARVB is predominantly expressed in tissues such as skeletal muscle, heart, and kidneys. Structurally, it contains two calponin homology (CH2) domains and a pleckstrin homology (PH) domain, enabling interactions with actin filaments and signaling proteins like paxillin, thereby regulating cytoskeletal dynamics and focal adhesion formation.
Functionally, PARVB mediates cell adhesion by bridging integrins and the actin cytoskeleton, influencing cell motility and tissue morphogenesis. It also modulates signaling pathways, including the PI3K/AKT and MAPK cascades, impacting cell survival, proliferation, and differentiation. Dysregulation of PARVB is linked to pathologies such as cancer metastasis, cardiovascular diseases, and neurological disorders, highlighting its dual role in physiological and pathological contexts.
Recombinant human PARVB protein, produced via genetic engineering in bacterial or mammalian systems, is widely used to study its molecular interactions, structural properties, and mechanistic contributions to diseases. Its applications span in vitro assays, structural studies, and functional analyses, offering insights into potential therapeutic targets. However, challenges in preserving post-translational modifications during recombinant production may necessitate optimization for specific experimental needs. Research on PARVB continues to unravel its complex roles in cellular homeostasis and disease progression.
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