| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCGF2 |
| Uniprot No | P35227 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344 aa |
| 活性数据 | MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA YPLTEVPNGS NEDRGEVLEQ EKGALSDDEI VSLSIEFYEG ARDRDEKKGP LENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEVLYEDE PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLAT VPTPSEGTNT SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP TPPSTASGAT TAANGGSLNC LQTPSSTSRG RKMTVNGAPV PPLT |
| 分子量 | 37.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人PCGF2蛋白的相关文献概述(信息基于公开研究整理，内容为模拟示例)：
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1. **文献名称**：PCGF2 regulates stem cell pluripotency through histone H3 lysine 27 trimethylation
**作者**：Zhang Y, et al.
**摘要**：本研究揭示了重组人PCGF2蛋白在胚胎干细胞中通过调控Polycomb repressive complex 1(PRC1)的活性，介导组蛋白H3K27me3修饰，进而维持干细胞多能性的分子机制。
2. **文献名称**：Recombinant human PCGF2 promotes osteogenic differentiation via BMP signaling pathway
**作者**：Li H, et al.
**摘要**：实验表明，重组人PCGF2蛋白可通过激活BMP/Smad信号通路增强间充质干细胞的成骨分化能力，为骨再生医学提供了潜在治疗靶点。
3. **文献名称**：Structural insights into PCGF2 ubiquitination activity in PRC1 assembly
**作者**：Matsui T, et al.
**摘要**：通过X射线晶体学解析重组人PCGF2蛋白结构，阐明了其RING结构域介导的泛素化功能对PRC1复合物组装及基因沉默活性的关键作用。
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**备注**：以上内容基于公开领域研究主题模拟创作，实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant PCGF2”、“PCGF2 function”获取。
Polycomb Group RING Finger Protein 2 (PCGF2), also known as Mel-18. is a member of the Polycomb group (PcG) protein family, which plays a critical role in epigenetic regulation of gene expression. PcG proteins are essential for maintaining transcriptional repression of specific genes, particularly those involved in cell differentiation, development, and identity. PCGF2 functions as a core component of the Polycomb Repressive Complex 1 (PRC1), contributing to chromatin compaction and monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin. This activity facilitates stable gene silencing, working in concert with PRC2-mediated H3K27me3 modifications to regulate stem cell pluripotency, tissue development, and oncogenesis.
Recombinant human PCGF2 protein is generated using biotechnology platforms (e.g., E. coli or mammalian expression systems) to produce a purified, biologically active form for research. Structural studies reveal that PCGF2 contains a conserved RING finger domain critical for E3 ubiquitin ligase activity, enabling PRC1 assembly and recruitment to chromatin. Its N-terminal and C-terminal regions mediate interactions with other PcG proteins, such as Ring1B, enhancing complex stability. Dysregulation of PCGF2 has been implicated in cancers and developmental disorders, driving interest in its molecular mechanisms. Recombinant PCGF2 is widely used to investigate PRC1-driven chromatin remodeling, cellular reprogramming, and therapeutic targeting of PcG-associated diseases.
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