| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF21A |
| Uniprot No | Q96BD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680 aa |
| 活性数据 | MELQTLQEAL KVEIQVHQKL VAQMKQDPQN ADLKKQLHEL QAKITALSEK QKRVVEQLRK NLIVKQEQPD KFQIQPLPQS ENKLQTAQQQ PLQQLQQQQQ YHHHHAQQSA AASPNLTASQ KTVTTASMIT TKTLPLVLKA ATATMPASVV GQRPTIAMVT AINSQKAVLS TDVQNTPVNL QTSSKVTGPG AEAVQIVAKN TVTLVQATPP QPIKVPQFIP PPRLTPRPNF LPQVRPKPVA QNNIPIAPAP PPMLAAPQLI QRPVMLTKFT PTTLPTSQNS IHPVRVVNGQ TATIAKTFPM AQLTSIVIAT PGTRLAGPQT VQLSKPSLEK QTVKSHTETD EKQTESRTIT PPAAPKPKRE ENPQKLAFMV SLGLVTHDHL EEIQSKRQER KRRTTANPVY SGAVFEPERK KSAVTYLNST MHPGTRKRGR PPKYNAVLGF GALTPTSPQS SHPDSPENEK TETTFTFPAP VQPVSLPSPT STDGDIHEDF CSVCRKSGQL LMCDTCSRVY HLDCLDPPLK TIPKGMWICP RCQDQMLKKE EAIPWPGTLA IVHSYIAYKA AKEEEKQKLL KWSSDLKQER EQLEQKVKQL SNSISKCMEM KNTILARQKE MHSSLEKVKQ LIRLIHGIDL SKPVDSEATV GAISNGPDCT PPANAATSTP APSPSSQSCT ANCNQGEETK |
| 分子量 | 74.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHF21A蛋白的3篇代表性文献(虚构示例，供格式参考)：
1. **文献名称**: *PHF21A regulates neuronal differentiation through histone demethylase interaction*
**作者**: Smith, J. et al.
**摘要**: 研究利用重组人PHF21A蛋白与去甲基化酶复合体(如LSD1)互作，证明其在神经元分化中通过染色质重塑调控靶基因表达，并解析其核定位功能域。
2. **文献名称**: *Structural analysis of PHF21A in chromatin remodeling complexes*
**作者**: Chen, L. & Wang, H.
**摘要**: 通过重组表达人PHF21A蛋白并解析其晶体结构，发现其PHD锌指结构域介导与组蛋白H3K4me2/3的结合，提示其在基因沉默中的表观遗传调控作用。
3. **文献名称**: *PHF21A mutations disrupt protein stability in neurodevelopmental disorders*
**作者**: González-Blanco, M. et al.
**摘要**: 利用重组PHF21A突变体进行生化分析，发现致病突变导致蛋白稳定性下降，影响其与CoREST复合物的结合，进而引发神经发育异常。
(注：以上文献为模拟示例，实际研究中需根据具体数据库检索。)
**Background of Recombinant Human PHF21A Protein**
Recombinant human PHF21A (PHD Finger Protein 21A) is a chromatin-associated protein involved in transcriptional regulation and epigenetic modifications. It belongs to the PHD zinc finger protein family, characterized by a plant homeodomain (PHD) that mediates interactions with histones, particularly recognizing methylated or unmethylated lysine residues. PHF21A is a core component of the BHC (BRAF-HDAC) complex, which links histone deacetylases (HDACs) to chromatin remodeling and gene repression.
Functionally, PHF21A plays a critical role in neural development, neuronal differentiation, and synapse formation by regulating chromatin structure and gene expression. Dysregulation or mutations in PHF21A are associated with neurodevelopmental disorders, such as Potocki-Shaffer syndrome and intellectual disabilities, highlighting its importance in brain function.
Recombinant PHF21A is produced via genetic engineering in bacterial or mammalian expression systems, enabling studies on its biochemical properties, protein-DNA interactions, and role in chromatin dynamics. It serves as a tool for investigating epigenetic mechanisms, identifying binding partners, and exploring therapeutic targets for neurodevelopmental diseases. Its applications extend to *in vitro* assays, structural studies, and drug screening platforms aimed at modulating chromatin-related pathways.
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