| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB28 |
| Uniprot No | P51157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-218 aa |
| 活性数据 | SDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMC |
| 分子量 | 24.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB28蛋白的3篇文献摘要(基于公开研究领域知识模拟，具体文献需核实)：
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1. **文献名称**：*Rab28 is involved in phagosome formation and protein trafficking in Dictyostelium*
**作者**：Horgan CP, et al.
**摘要**：研究分析了RAB28在细胞吞噬体和膜运输中的作用，通过重组表达标记蛋白，发现其参与溶酶体途径调控，并影响细胞内膜结构动态。
2. **文献名称**：*RAB28 modulates cell proliferation and migration in colorectal cancer via PI3K/AKT pathway*
**作者**：Zhang Y, et al.
**摘要**：利用重组RAB28蛋白进行体外功能实验，发现其通过调控PI3K/AKT信号通路影响结直肠癌细胞的增殖和迁移能力。
3. **文献名称**：*Functional characterization of RAB28 in cilia-associated protein transport*
**作者**：Moriyama K, et al.
**摘要**：通过重组RAB28和活细胞成像技术，揭示了其在纤毛形成中的关键作用，证明其与IFT颗粒相互作用并影响纤毛相关蛋白运输。
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注：上述内容为领域知识归纳示例，实际文献需通过PubMed等平台检索确认。
**Background of Recombinant Human RAB28 Protein**
RAB28. a member of the Ras-associated binding (RAB) protein family, is a small GTPase involved in intracellular vesicular trafficking and membrane dynamics. As part of the RAB GTPase superfamily, it cycles between active GTP-bound and inactive GDP-bound states, regulating cargo transport, organelle positioning, and signal transduction. Human RAB28 is encoded by the *RAB28* gene located on chromosome 4p15.2 and is ubiquitously expressed, with notable roles in cilia function, photoreceptor maintenance, and autophagy. Studies suggest its involvement in specialized cellular processes, such as lipid droplet formation and lysosomal degradation, though its precise mechanisms remain under investigation.
Dysregulation of RAB28 has been linked to genetic disorders, including autosomal recessive cone-rod dystrophy, highlighting its importance in retinal homeostasis. Additionally, RAB28 may interact with proteins like TBC1D7. a regulator of mTORC1 signaling, implying potential cross-talk with metabolic pathways. Recombinant human RAB28 protein, typically produced using bacterial or mammalian expression systems, enables in vitro studies to dissect its molecular interactions, post-translational modifications, and GTPase activity. Purified recombinant RAB28 serves as a tool for structural analysis, antibody development, and screening for binding partners or inhibitors. Despite progress, gaps persist in understanding its tissue-specific roles and disease-associated variants. Ongoing research aims to elucidate its contribution to cellular physiology and pathophysiology, offering avenues for therapeutic targeting in ciliopathies, neurodegenerative diseases, or cancer.
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