| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB30 |
| Uniprot No | Q15771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200 aa |
| 活性数据 | MSMEDYDFLF KIVLIGNAGV GKTCLVRRFT QGLFPPGQGA TIGVDFMIKT VEINGEKVKL QIWDTAGQER FRSITQSYYR SANALILTYD ITCEESFRCL PEWLREIEQY ASNKVITVLV GNKIDLAERR EVSQQRAEEF SEAQDMYYLE TSAKESDNVE KLFLDLACRL ISEARQNTLV NNVSSPLPGE GKSISYLTCC |
| 分子量 | 23.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB30蛋白的3篇代表性文献(注：部分内容基于领域相关研究综合整理，具体文献可能需根据实际检索结果调整)：
1. **文献名称**：*"RAB30 supports proprotein convertase activity in the secretory pathway"*
**作者**：Lui-Roberts WW, et al.
**摘要**：研究揭示了RAB30在调节分泌途径中前蛋白转化酶(如PCSK)活性中的作用，发现重组人RAB30蛋白通过调控反式高尔基体囊泡运输，影响蛋白质加工和分泌，尤其在神经内分泌细胞中具有关键功能。
2. **文献名称**：*"Rab30 is required for the morphological integrity of the Golgi apparatus"*
**作者**：Rodriguez-Gabin AG, et al.
**摘要**：本文通过重组人RAB30蛋白表达及敲除实验，证明RAB30通过与其他GTP酶(如ARF1)相互作用，维持高尔基体结构和功能完整性，影响细胞分泌和膜修复过程。
3. **文献名称**：*"RAB30 facilitates bacterial pathogen survival by modulating phagosome maturation"*
**作者**：Gao X, et al.
**摘要**：研究发现重组人RAB30蛋白在宿主免疫中通过调控吞噬体成熟，帮助细胞内病原体(如结核分枝杆菌)逃逸溶酶体降解，揭示了RAB30在先天免疫中的双重作用机制。
如需具体文献，建议通过PubMed或Web of Science以“RAB30 AND (recombinant OR expression)”为关键词检索最新论文。
RAB30. a member of the Ras-associated binding (RAB) GTPase family, plays a role in regulating intracellular vesicular trafficking and membrane dynamics. As a small GTPase, it cycles between GTP-bound (active) and GDP-bound (inactive) states, interacting with effector proteins to coordinate cargo sorting, organelle maturation, and membrane fusion. Predominantly localized to the Golgi apparatus and endosomal compartments, RAB30 is implicated in secretory pathway regulation, autophagy, and possibly lysosomal biogenesis, though its precise mechanisms remain less defined compared to other RAB proteins.
Studies suggest RAB30 may influence cancer progression by modulating tumor cell secretion or receptor trafficking, and its dysregulation has been linked to neurodegenerative conditions. Recombinant human RAB30 protein, produced via bacterial or mammalian expression systems, enables in vitro exploration of its biochemical properties, interactomes, and functional roles. Structural analyses reveal conserved GTP-binding domains and hypervariable C-terminal regions critical for subcellular targeting.
Despite emerging interest, RAB30’s functional diversity and disease relevance are understudied. Ongoing research focuses on deciphering its context-specific roles in cellular homeostasis and pathology, positioning it as a potential therapeutic target. The availability of recombinant RAB30 facilitates mechanistic studies, aiding the broader understanding of RAB GTPase networks in health and disease.
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