| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB2 |
| Uniprot No | P61019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212 aa |
| 活性数据 | MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNATHAGNQGGQQAGGGCC |
| 分子量 | 49.06 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB2蛋白研究的模拟参考文献示例(注：以下为学术示例，实际文献需通过数据库检索获取)：
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1. **标题**: "Expression and Functional Characterization of Recombinant Human RAB2 GTPase"
**作者**: Zhang Y, et al.
**摘要**: 该研究成功在大肠杆菌中表达了重组人RAB2蛋白，并对其GTP水解活性和核苷酸结合特性进行了分析，揭示了RAB2在体外条件下与效应蛋白相互作用的依赖性。
2. **标题**: "RAB2 Regulates ER-Golgi Trafficking through Interaction with COPI Complex"
**作者**: Smith JL, et al.
**摘要**: 通过免疫共沉淀和RNA干扰实验，发现重组RAB2与COPI囊泡复合物结合，调控内质网至高尔基体的逆向运输，敲低RAB2导致分泌通路紊乱。
3. **标题**: "Structural Insights into RAB2 Activation by GTP Loading"
**作者**: Jones M, et al.
**摘要**: 利用X射线晶体学解析了RAB2在GDP/GTP结合状态下的三维结构，揭示了其构象变化如何影响效应蛋白的结合位点暴露，为靶向RAB2的药物设计提供结构基础。
4. **标题**: "RAB2 Overexpression Promotes Tumor Metastasis via Autophagy Modulation"
**作者**: Lee S, et al.
**摘要**: 在癌细胞模型中，重组RAB2过表达通过增强自噬小体形成促进细胞侵袭，提示RAB2可能作为癌症治疗的潜在靶点。
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**建议**：实际文献请通过 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Google Scholar** 搜索关键词 "recombinant RAB2" / "human RAB2 function" 获取最新研究。若需全文，可使用机构数据库(如Web of Science)或联系作者索取。
Recombinant human RAB2 protein belongs to the Ras-associated binding (RAB) GTPase family, a critical subgroup of small GTPases involved in intracellular membrane trafficking and vesicle transport. RAB2 specifically localizes to the Golgi apparatus and endoplasmic reticulum (ER), playing a pivotal role in regulating Golgi-to-ER retrograde transport and maintaining Golgi structure. Structurally, it contains conserved GTP-binding domains and effector interaction regions, cycling between active GTP-bound and inactive GDP-bound states to coordinate cargo sorting, membrane fusion, and cytoskeletal interactions.
Studies link RAB2 dysfunction to disrupted secretion, autophagy, and organelle dynamics, contributing to pathological conditions such as neurodegenerative disorders, cancer, and infectious diseases. For instance, RAB2 interacts with pathogens like *Legionella pneumophila*, which hijack its trafficking mechanisms for intracellular survival. Recombinant RAB2 proteins, typically produced via bacterial or mammalian expression systems, enable in vitro studies of its biochemical properties, post-translational modifications (e.g., prenylation), and interactions with molecular partners. These tools are vital for dissecting RAB2’s regulatory networks in membrane trafficking and exploring therapeutic strategies targeting RAB-mediated pathways. Current research also investigates its role in unconventional secretion and stress responses, highlighting its broader impact on cellular homeostasis.
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