| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABEP2 |
| Uniprot No | Q9H5N1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-569 aa |
| 活性数据 | AAAAPVAAD DDERRRRPGA ALEDSRSQEG ANGEAESGEL SRLRAELAGA LAEMETMKAV AEVSESTKAE AVAAVQRQCQ EEVASLQAIL KDSISSYEAQ ITALKQERQQ QQQDCEEKER ELGRLKQLLS RAYPLDSLEK QMEKAHEDSE KLREIVLPME KEIEELKAKL LRAEELIQEI QRRPRHAPSL HGSTELLPLS RDPSPPLEPL EELSGDGGPA AEAFAHNCDD SASISSFSLG GGVGSSSSLP QSRQGLSPEQ EETASLVSTG TLVPEGIYLP PPGYQLVPDT QWEQLQTEGR QLQKDLESVS RERDELQEGL RRSNEDCAKQ MQVLLAQVQN SEQLLRTLQG TVSQAQERVQ LQMAELVTTH KCLHHEVKRL NEENQGLRAE QLPSSAPQGS QQEQGEEESL PSSVPELQQL LCCTRQEARA RLQAQEHGAE RLRIEIVTLR EALEEETVAR ASLEGQLRVQ REETEVLEAS LCSLRTEMER VQQEQSKAQL PDLLSEQRAK VLRLQAELET SEQVQRDFVR LSQALQVRLE RIRQAETLEQ VRSIMDEAPL TDVRDIKDT |
| 分子量 | 63.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABEP2蛋白的假设参考文献示例，基于该领域常见研究方向整理：
1. **文献名称**：**"RABEP2 regulates vesicular trafficking through Rab GTPase interaction in human cells"**
**作者**：Smith A et al.
**摘要**：本研究通过重组表达人RABEP2蛋白，结合细胞功能实验，揭示了其通过与Rab5/Rab4等GTP酶结合调控内体膜运输的机制，证明其在受体回收通路中的关键作用。
2. **文献名称**：**"Structural insights into RABEP2's effector domain via recombinant protein crystallography"**
**作者**：Lee C et al.
**摘要**：利用重组人RABEP2蛋白进行X射线晶体学分析，首次解析了其羧酸端效应结构域的原子级结构，阐明了其与Rab蛋白特异性结合的关键氨基酸残基。
3. **文献名称**：**"Recombinant RABEP2 expression links genetic variants to impaired glucose metabolism"**
**作者**：Zhang Y et al.
**摘要**：通过构建携带糖尿病相关突变体的重组RABEP2蛋白，发现特定位点变异导致其与Rab7A结合能力下降，进而影响胰岛β细胞囊泡运输和胰岛素分泌功能。
4. **文献名称**：**"RABEP2 knockdown and recombinant rescue in neuronal models of neurodevelopmental disorders"**
**作者**：Johnson R et al.
**摘要**：在神经元细胞中，外源添加重组RABEP2蛋白可逆转由基因沉默引起的突触囊泡运输缺陷，提示其在神经发育疾病中的潜在治疗价值。
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注：上述文献为假设示例，实际研究中建议通过 **PubMed/Google Scholar** 以关键词 **"recombinant RABEP2"** 或 **"RABEP2 protein function"** 检索最新实证研究。
Recombinant human RABEP2 (Rab GTPase-binding effector protein 2) is a protein encoded by the RABEP2 gene, belonging to the Rab effector family. It interacts with Rab GTPases, key regulators of membrane trafficking and vesicular transport. Structurally, RABEP2 contains an N-terminal BAR (Bin/Amphiphysin/Rvs) domain implicated in membrane curvature sensing and a C-terminal region with variable motifs for protein-protein interactions. Its primary function involves modulating endosomal dynamics, particularly in receptor recycling and endosome-lysosome trafficking. Studies suggest its role in coordinating Rab4/Rab5-dependent pathways, influencing cargo sorting and intracellular signaling.
The recombinant form is typically expressed in bacterial or mammalian systems for biochemical and cellular studies, enabling exploration of its molecular mechanisms. Dysregulation of RABEP2 has been loosely associated with cancers, neurodevelopmental disorders, and metabolic diseases, though its pathophysiological relevance remains under investigation. Its recombinant protein serves as a critical tool for dissecting Rab-mediated trafficking networks and screening therapeutic targets. Current research emphasizes its regulatory crosstalk with other Rab effectors and potential applications in drug delivery engineering.
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