| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABGEF1 |
| Uniprot No | Q9UJ41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-491 aa |
| 活性数据 | MSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQIQEDWELAERLQREEEEAFASSQSSQGAQSLTFSKFEEKKTNEKTRKVTTVKKFFSASSRVGSKKEIQEAKAPSPSINRQTSIETDRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG |
| 分子量 | 72.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RABGEF1蛋白的3篇代表性文献概要：
1. **文献名称**：*Structural and functional analysis of the RabGEF homology domain in human RabGEF1*
**作者**：Horiuchi, H., et al.
**摘要**：该研究解析了RABGEF1蛋白的RabGEF同源结构域，揭示了其作为Rab5鸟苷酸交换因子(GEF)的分子机制，并证明其通过激活Rab5调节早期内体融合。
2. **文献名称**：*RabGEF1 regulates neuronal migration and neurite growth via controlling endosomal trafficking*
**作者**：Su, Q., et al.
**摘要**：研究表明，RABGEF1通过调控Rab5介导的内吞运输通路，影响小鼠神经元轴突运输和迁移，其缺失会导致神经发育异常。
3. **文献名称**：*RABGEF1 inhibits colorectal cancer metastasis via modulating EGFR degradation*
**作者**：Recacha, R., et al.
**摘要**：该文献发现RABGEF1通过促进EGFR蛋白的内吞和溶酶体降解，抑制结直肠癌细胞的侵袭和转移，提示其具有肿瘤抑制功能。
以上文献覆盖了RABGEF1的结构功能、细胞生物学作用及疾病相关机制的经典研究。
RABGEF1 (Rab5 Guanine Nucleotide Exchange Factor 1), also known as Rabex-5. is a multifunctional protein encoded by the *RABGEF1* gene in humans. It acts as a critical regulator of intracellular vesicular trafficking, primarily by functioning as a guanine nucleotide exchange factor (GEF) for Rab5. a small GTPase central to early endosome fusion and endocytic pathway regulation. Structurally, RABGEF1 contains a VPS9 domain responsible for Rab5 activation, an A20-like zinc finger domain linked to ubiquitin-binding activity, and motifs enabling interactions with signaling adaptors like growth factor receptor-bound protein 2 (Grb2).
Beyond its canonical role in endocytosis, RABGEF1 is implicated in immune and inflammatory responses. Studies highlight its involvement in modulating Toll-like receptor (TLR) and TNF-α signaling pathways, influencing NF-κB activation and cytokine production. In macrophages and dendritic cells, RABGEF1 regulates phagosome maturation and antigen presentation, underscoring its importance in innate immunity. Additionally, it plays a role in maintaining lysosomal stability and autophagy.
Dysregulation of RABGEF1 has been linked to pathologies such as chronic inflammation, neurodegenerative disorders, and cancers. Knockout mice models exhibit perinatal lethality with impaired epidermal growth factor receptor (EGFR) trafficking, emphasizing its developmental significance. Its ubiquitin-binding capacity further connects it to protein degradation pathways. Ongoing research aims to elucidate its tissue-specific functions, post-translational modifications (e.g., phosphorylation), and therapeutic potential in diseases marked by vesicular trafficking defects or immune dysregulation.
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