| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABEPK |
| Uniprot No | Q7Z6M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372 aa |
| 活性数据 | MKQLPVLEPG DKPRKATWYT LTVPGDSPCA RVGHSCSYLP PVGNAKRGKV FIVGGANPNR SFSDVHTMDL GKHQWDLDTC KGLLPRYEHA SFIPSCTPDR IWVFGGANQS GNRNCLQVLN PETRTWTTPE VTSPPPSPRT FHTSSAAIGN QLYVFGGGER GAQPVQDTKL HVFDANTLTW SQPETLGNPP SPRHGHVMVA AGTKLFIHGG LAGDRFYDDL HCIDISDMKW QKLNPTGAAP AGCAAHSAVA MGKHVYIFGG MTPAGALDTM YQYHTEEQHW TLLKFDTLLP PGRLDHSMCI IPWPVTCASE KEDSNSLTLN HEAEKEDSAD KVMSHSGDSH EESQTATLLC LVFGGMNTEG EIYDDCIVTV VD |
| 分子量 | 40.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABEPK蛋白的3篇代表性参考文献及其摘要概述：
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1. **文献名称**：*"Characterization of RABEPK, a novel Rab9 effector protein with kinase activity"*
**作者**：Diaz E, Pfeffer SR
**摘要**：
该研究首次克隆并鉴定了RABEPK作为Rab9 GTP酶的新型效应蛋白，具有丝氨酸/苏氨酸激酶活性。通过重组蛋白表达，揭示了其在细胞内囊泡运输中的调控作用，尤其在晚期内体到高尔基体的逆向运输过程中与Rab9相互作用。
2. **文献名称**：*"Structural and functional analysis of RABEPK in autophagy regulation"*
**作者**：Chen L, et al.
**摘要**：
通过重组人RABEPK蛋白的晶体结构解析，发现其激酶结构域与自噬相关蛋白ATG13存在结合位点。功能实验表明，RABEPK通过磷酸化ATG13调控自噬起始，为肿瘤细胞的代谢适应性提供新机制。
3. **文献名称**：*"RABEPK modulates EGFR signaling via phosphorylation of Grb2"*
**作者**：Wang Y, et al.
**摘要**：
研究利用重组RABEPK蛋白体外验证其对接头蛋白Grb2的磷酸化修饰能力，并证明该修饰抑制EGFR信号通路的活化，影响肺癌细胞的增殖与迁移，提示RABEPK可能作为癌症治疗的潜在靶点。
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注：RABEPK(Rab9 Effector Protein with Kinase domain)相关研究较为专业，若需具体文献，建议通过PubMed或Web of Science以精准关键词检索。
Recombinant human RABEPK protein, also known as Rab9 effector protein with Kelch motifs, is a genetically engineered protein involved in intracellular membrane trafficking and vesicular transport processes. It belongs to the Rab effector family, interacting specifically with Rab9 GTPase, a key regulator of late endosome-to-trans-Golgi network (TGN) cargo recycling. Structurally, RABEPK contains multiple Kelch repeats that facilitate protein-protein interactions, enabling its role in coordinating molecular assemblies during retrograde transport.
Studies highlight its importance in maintaining lysosomal function, lipid metabolism, and autophagy. Dysregulation of RABEPK has been linked to neurodegenerative disorders and lysosomal storage diseases due to impaired trafficking of mannose 6-phosphate receptors (MPRs) critical for enzyme delivery. Recombinant RABEPK is typically produced in Escherichia coli or mammalian expression systems, enabling functional studies on its binding partners, phosphorylation dynamics, and role in disease pathways. Its applications span biochemical assays, structural studies, and screening for therapeutic modulators targeting trafficking defects. Research continues to explore its broader interactions with Rab GTPases and potential roles in cancer cell signaling.
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