| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABGGTB |
| Uniprot No | P53611 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-331 aa |
| 活性数据 | GTPQKDVII KSDAPDTLLL EKHADYIASY GSKKDDYEYC MSEYLRMSGI YWGLTVMDLM GQLHRMNREE ILAFIKSCQH ECGGISASIG HDPHLLYTLS AVQILTLYDS INVIDVNKVV EYVKGLQKED GSFAGDIWGE IDTRFSFCAV ATLALLGKLD AINVEKAIEF VLSCMNFDGG FGCRPGSESH AGQIYCCTGF LAITSQLHQV NSDLLGWWLC ERQLPSGGLN GRPEKLPDVC YSWWVLASLK IIGRLHWIDR EKLRNFILAC QDEETGGFAD RPGDMVDPFH TLFGIAGLSL LGEEQIKPVN PVFCMPEEVL QRVNVQPELV S |
| 分子量 | 36.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABGGTB蛋白的3篇示例参考文献(基于领域知识推测，建议通过学术数据库验证)：
1. **文献名称**：*"Characterization of Recombinant Human Rab Geranylgeranyltransferase Beta Subunit (RABGGTB) in Enzymatic Prenylation"*
**作者**：Smith A, et al.
**摘要**：研究重组表达的RABGGTB蛋白与其α亚基复合物的酶活性，揭示其在RAB GTPase异戊烯化中的底物特异性及催化机制。
2. **文献名称**：*"Expression and Purification of Functional RABGGTB in Escherichia coli for Structural Studies"*
**作者**：Chen L, et al.
**摘要**：开发了一种在大肠杆菌中高效表达重组RABGGTB的方法，通过纯化获得高纯度蛋白，并用于后续的X射线晶体学分析。
3. **文献名称**：*"Role of RABGGTB in Vesicular Trafficking: Insights from Recombinant Protein Knockdown Models"*
**作者**：Wang Y, et al.
**摘要**：利用重组RABGGTB蛋白构建体外细胞模型，证实其在囊泡运输中的关键作用，并发现与神经退行性疾病的潜在关联。
**提示**：以上为示例，具体文献需通过PubMed、Google Scholar等平台检索关键词“RABGGTB recombinant”“RAB geranylgeranyltransferase beta”获取。
Recombinant human RABGGTB (RAB geranylgeranyltransferase subunit beta) is a protein critical for intracellular vesicular trafficking and membrane dynamics. As a component of the heterodimeric RAB geranylgeranyltransferase (RABGGTase) complex, RABGGTB works in conjunction with the alpha subunit (RABGGTA) to catalyze the post-translational addition of geranylgeranyl lipid groups to RAB GTPases. This lipid modification, known as prenylation, is essential for anchoring RAB proteins to membranes, enabling their role in regulating vesicle formation, transport, and fusion processes. Dysregulation of RABGGTB-mediated prenylation has been linked to cellular trafficking disorders, neurodegenerative diseases, and cancer progression.
The recombinant form of RABGGTB is typically produced in bacterial or mammalian expression systems for research applications. Its production allows detailed biochemical studies, including enzyme kinetics, structural analysis (e.g., X-ray crystallography), and inhibitor screening to develop therapeutic agents targeting RAB-dependent pathways. Recombinant RABGGTB also serves as a tool to investigate prenylation defects in genetic disorders like choroideremia and Hermansky-Pudlak syndrome. Recent studies emphasize its potential as a biomarker in certain cancers due to altered expression patterns in tumor cells. Ongoing research focuses on understanding its allosteric regulation within the RABGGTase complex and exploring isoform-specific functions in different cellular compartments.
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